Synthesis and MALDI-TOF MS Analysis of Protected Oligosaccharide Components of N-Glycoproteins

Karoly Agoston, Gyongyi Gyemant, Laszlo Kalmar, Janos Kerekgyarto, Zoltan Szurmai, Boglarka Donczo, A. Guttman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Essential components of N-glycoproteins were synthesized in octyl glycoside form starting from 3-O-allyl-D-glucose derivative. The β-mannosidic linkage was formed by the oxidation reduction method. MALDI-TOF mass spectrometry and its post-source decay (PSD) mode were used for identification and structural elucidation of protected synthetic oligosaccharides related to N-glycoproteins. Most fragments, identified in the PSD spectra, were products of the cleavage of glycosidic bonds.

Original languageEnglish
Pages (from-to)326-343
Number of pages18
JournalJournal of Carbohydrate Chemistry
Volume33
Issue number6
DOIs
Publication statusPublished - Jul 24 2014

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Oligosaccharides
Glycoproteins
Glycosides
Oxidation-Reduction
Mass spectrometry
Mass Spectrometry
Derivatives
Glucose

Keywords

  • MALDI-TOF MS
  • N-Glycoprotein
  • Oligosaccharide synthesis
  • Post-source decay (PSD)
  • β-Mannoside

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Cite this

Synthesis and MALDI-TOF MS Analysis of Protected Oligosaccharide Components of N-Glycoproteins. / Agoston, Karoly; Gyemant, Gyongyi; Kalmar, Laszlo; Kerekgyarto, Janos; Szurmai, Zoltan; Donczo, Boglarka; Guttman, A.

In: Journal of Carbohydrate Chemistry, Vol. 33, No. 6, 24.07.2014, p. 326-343.

Research output: Contribution to journalArticle

Agoston, Karoly ; Gyemant, Gyongyi ; Kalmar, Laszlo ; Kerekgyarto, Janos ; Szurmai, Zoltan ; Donczo, Boglarka ; Guttman, A. / Synthesis and MALDI-TOF MS Analysis of Protected Oligosaccharide Components of N-Glycoproteins. In: Journal of Carbohydrate Chemistry. 2014 ; Vol. 33, No. 6. pp. 326-343.
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