Synthesis and application of novel bifunctional spin labels

Ralf M. Lösel, Reinhard Philipp, T. Kalai, K. Hideg, Wolfgang E. Trommer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The synthesis of new bifunctional spin-labeled cross-linking reagents is described. Covalent attachment to papain was achieved via a thiol-specific thiosulfonate residue and, for the second anchor point, via a nonspecific photoreactive azido function. The thiosulfonate formed a reversible disulfide linkage, which could be cleaved again reductively by dithiothreitol. The spin label, a pyrroline-1-oxyl radical, was highly immobilized after attachment to papain by both functional groups and showed little if any relative motion with respect to the protein.

Original languageEnglish
Pages (from-to)578-582
Number of pages5
JournalBioconjugate Chemistry
Volume10
Issue number4
DOIs
Publication statusPublished - Jul 1999

Fingerprint

Papain
Spin Labels
Labels
Cross-Linking Reagents
Dithiothreitol
Anchors
Sulfhydryl Compounds
Disulfides
Functional groups
Proteins
pyrroline

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Synthesis and application of novel bifunctional spin labels. / Lösel, Ralf M.; Philipp, Reinhard; Kalai, T.; Hideg, K.; Trommer, Wolfgang E.

In: Bioconjugate Chemistry, Vol. 10, No. 4, 07.1999, p. 578-582.

Research output: Contribution to journalArticle

Lösel, Ralf M. ; Philipp, Reinhard ; Kalai, T. ; Hideg, K. ; Trommer, Wolfgang E. / Synthesis and application of novel bifunctional spin labels. In: Bioconjugate Chemistry. 1999 ; Vol. 10, No. 4. pp. 578-582.
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