Synthesis and application of a Sec2-containing oligopeptide for method evaluation purposes in selenium speciation

Mihály Dernovics, Andrea Vass, Anikó Németh, Anna Magyar

Research output: Contribution to journalArticle

4 Citations (Scopus)


Sec2-containing oligopeptide was synthesized directly from Sec2 with the traditional liquid phase peptide synthesis without addressing the usually applied and complex solid phase (SPPS) protocol driving through a protected Sec residue and site-oriented oxidation into a diselenide bridge. Effective solubilization of Sec2 in dimethylformamide and its pH-controlled access to pentachlorophenol-activated peptides to couple with were of crucial importance to achieve good yield (>50%) of synthesis, monitored by HPLC-UV, SEC-ICP-MS and HPLC-ESI-MS techniques. To demonstrate the possible application of the new compound, (Boc-GGFG)-Sec2-(Boc-GGFG) (m/z 1173.3, [MH]), it was utilized to compare the effect of the two most addressed sample preparation techniques, i.e., methanesulphonic acid (MSA) based digestion and proteolytic digestion with protease XIV, on the Sec residue. The study revealed that the use of MSA resulted in the decomposition of Sec even after derivatization with iodoacetamide.

Original languageEnglish
Pages (from-to)186-193
Number of pages8
Publication statusPublished - Sep 15 2012


  • Oligopeptide
  • Selenium
  • Selenocysteine
  • Selenocystine
  • Speciation

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Synthesis and application of a Sec<sub>2</sub>-containing oligopeptide for method evaluation purposes in selenium speciation'. Together they form a unique fingerprint.

  • Cite this