Synthesis and application of a novel, crystalline phosphoramidite monomer with thiol terminus, suitable for the synthesis of DNA conjugates

Zoltán Kupihár, Zoltán Schmél, Zoltán Kele, Botond Penke, Lajos Kovács

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14 Citations (Scopus)

Abstract

A new, crystalline 5′-thiol modifier phosphoramidite monomer (3), suitable for DNA synthesis, has been prepared. This monomer has been built into an oligonucleotide using the standard protocol. After cleavage, purification and removal of the trityl group with Ag+, a free 5′-thiol terminal oligonucleotide (15) has been obtained which was subsequently coupled to a cysteine derivative via a disulfide bridge to afford conjugate 16.

Original languageEnglish
Pages (from-to)1241-1247
Number of pages7
JournalBioorganic and Medicinal Chemistry
Volume9
Issue number5
DOIs
Publication statusPublished - May 1 2001

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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