Synthesis and analysis of peptide nucleic acid oligomers using Fmoc/acyl-protected monomers

Györgyi Kovács, Zoltán Timár, Zoltán Kupihár, Z. Kele, L. Kovács

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The optimization of PNA oligomer synthesis has been accomplished employing Fmoc/acyl-protected monomers on TentaGel™ and Wang resins. Among the tested activating agents (CMP, BET, HATU) the latter was of choice in solid phase syntheses. "Leakage" of TentaGel™ resin greatly hampers the solution and MS analyses. Synthesis and acyl group deprotection steps have been separately examined using Wang resin. Optimal conditions also worked well on the CPG support. HPLC and MS analyses of the PNA oligomers were carried out under various conditions.

Original languageEnglish
Pages (from-to)1266-1270
Number of pages5
JournalJournal of the Chemical Society, Perkin Transactions 1
Issue number10
Publication statusPublished - 2002

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Peptide Nucleic Acids
Oligomers
Monomers
Cytidine Monophosphate
Leakage (fluid)
Tentagel resin
Wang resin

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Synthesis and analysis of peptide nucleic acid oligomers using Fmoc/acyl-protected monomers. / Kovács, Györgyi; Timár, Zoltán; Kupihár, Zoltán; Kele, Z.; Kovács, L.

In: Journal of the Chemical Society, Perkin Transactions 1, No. 10, 2002, p. 1266-1270.

Research output: Contribution to journalArticle

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