Synovial fluid exoglycosidases are predictors of rheumatoid arthritis and are effective in cartilage glycosaminoglycan depletion

Zsuzsanna Ortutay, Anna Polgá, Béla Gömör, P. Géher, Tamás Lakatos, Tibor T. Glant, Renate E. Gay, Steffen Gay, E. Pállinger, Csaba Farkas, Éva Farkas, L. Tóthfalusi, K. Kocsis, A. Falus, E. Búzás

Research output: Contribution to journalArticle

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Abstract

Objective. To analyze enzymes involved in joint damage by simultaneous investigation of glycosidases and matrix metalloproteinases (MMPs) in patients with various joint diseases. Methods. Activities of glycosidases (β-D-glucuronidase, β-D-N-acetyl-glucosaminidase, β-D-N-acetyl-galactosaminidase, β-D-galactosidase, and α-D-mannosidase) were tested at an acidic pH as well as at the original pH of the synovial fluid (SF) samples in parallel with activities of MMP-1 and MMP-9. Results. Patients with rheumatoid arthritis (RA) were characterized by significantly elevated activities of β-D-glucuronidase and β-D-N-acetyl-glucosaminidase in SF compared with patients with osteoarthritis, seronegative spondylarthritis, or acute sports injury. To select the best predictor for distinguishing among patient groups, a stepwise logistic regression analysis was performed; the strongest association was found to be between RA and β-D-glucuronidase/β-D-N-acetyl-glucosaminidase activities (measured at the pH of the SF). Further, a significant correlation was observed between the activity of SF β-D-N-acetyl-glucosaminidase and the level of rheumatoid factor. In vitro digestion of human hyaline cartilage samples revealed that the dominant glycosidases, alone or in combination with MMPs, proved to be effective in depleting glycosaminoglycans (GAGs) from cartilage. Conclusion. These results suggest that exoglycosidases, which are present in the SF of RA patients, may contribute to the depletion of GAGs from cartilage and thereby facilitate the invasion of synovial cells and their attachment to cartilage in RA.

Original languageEnglish
Pages (from-to)2163-2172
Number of pages10
JournalArthritis and Rheumatism
Volume48
Issue number8
DOIs
Publication statusPublished - Aug 1 2003

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Hexosaminidases
Glycoside Hydrolases
Synovial Fluid
Glycosaminoglycans
Cartilage
Rheumatoid Arthritis
Glucuronidase
Matrix Metalloproteinases
Spondylarthritis
Mannosidases
Galactosidases
Hyaline Cartilage
Athletic Injuries
Matrix Metalloproteinase 1
Joint Diseases
Rheumatoid Factor
Matrix Metalloproteinase 9
Osteoarthritis
Digestion
Joints

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Synovial fluid exoglycosidases are predictors of rheumatoid arthritis and are effective in cartilage glycosaminoglycan depletion. / Ortutay, Zsuzsanna; Polgá, Anna; Gömör, Béla; Géher, P.; Lakatos, Tamás; Glant, Tibor T.; Gay, Renate E.; Gay, Steffen; Pállinger, E.; Farkas, Csaba; Farkas, Éva; Tóthfalusi, L.; Kocsis, K.; Falus, A.; Búzás, E.

In: Arthritis and Rheumatism, Vol. 48, No. 8, 01.08.2003, p. 2163-2172.

Research output: Contribution to journalArticle

Ortutay, Zsuzsanna ; Polgá, Anna ; Gömör, Béla ; Géher, P. ; Lakatos, Tamás ; Glant, Tibor T. ; Gay, Renate E. ; Gay, Steffen ; Pállinger, E. ; Farkas, Csaba ; Farkas, Éva ; Tóthfalusi, L. ; Kocsis, K. ; Falus, A. ; Búzás, E. / Synovial fluid exoglycosidases are predictors of rheumatoid arthritis and are effective in cartilage glycosaminoglycan depletion. In: Arthritis and Rheumatism. 2003 ; Vol. 48, No. 8. pp. 2163-2172.
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abstract = "Objective. To analyze enzymes involved in joint damage by simultaneous investigation of glycosidases and matrix metalloproteinases (MMPs) in patients with various joint diseases. Methods. Activities of glycosidases (β-D-glucuronidase, β-D-N-acetyl-glucosaminidase, β-D-N-acetyl-galactosaminidase, β-D-galactosidase, and α-D-mannosidase) were tested at an acidic pH as well as at the original pH of the synovial fluid (SF) samples in parallel with activities of MMP-1 and MMP-9. Results. Patients with rheumatoid arthritis (RA) were characterized by significantly elevated activities of β-D-glucuronidase and β-D-N-acetyl-glucosaminidase in SF compared with patients with osteoarthritis, seronegative spondylarthritis, or acute sports injury. To select the best predictor for distinguishing among patient groups, a stepwise logistic regression analysis was performed; the strongest association was found to be between RA and β-D-glucuronidase/β-D-N-acetyl-glucosaminidase activities (measured at the pH of the SF). Further, a significant correlation was observed between the activity of SF β-D-N-acetyl-glucosaminidase and the level of rheumatoid factor. In vitro digestion of human hyaline cartilage samples revealed that the dominant glycosidases, alone or in combination with MMPs, proved to be effective in depleting glycosaminoglycans (GAGs) from cartilage. Conclusion. These results suggest that exoglycosidases, which are present in the SF of RA patients, may contribute to the depletion of GAGs from cartilage and thereby facilitate the invasion of synovial cells and their attachment to cartilage in RA.",
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AU - Ortutay, Zsuzsanna

AU - Polgá, Anna

AU - Gömör, Béla

AU - Géher, P.

AU - Lakatos, Tamás

AU - Glant, Tibor T.

AU - Gay, Renate E.

AU - Gay, Steffen

AU - Pállinger, E.

AU - Farkas, Csaba

AU - Farkas, Éva

AU - Tóthfalusi, L.

AU - Kocsis, K.

AU - Falus, A.

AU - Búzás, E.

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