Synergistic effects of thalidomide and poly(ADP-ribose) polymerase inhibition on type II collagen-induced arthritis in mice

Hans Kröger, Ralf Miesel, Annette Dietrich, Manuela Ohde, E. Rajnavolgyi, Heinrich Ockenfels

Research output: Contribution to journalArticle

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Abstract

The present study investigates synergistic effects of the TNF-α inhibitor thalidomide and the poly(ADP-ribose) polymerase (PARP)-inhibitor nicotinic acid amide (NAA) in male DBA/1 hybrid mice suffering from type II collagen-induced arthritis. Parameters including the arthritis index, chemiluminescence and anti-collagen antibody titers were used for the assessment of disease activity: The disease courses demonstrated clearly an inhibitory effect of thalidomide. NAA inhibited established collagen arthritis in a dose-dependent manner. The combined application of thalidomide and NAA caused a powerful synergistic inhibition of arthritis. Furthermore, thalidomide and NAA were tested ex vivo for their inhibition of the NADPH oxidase-dependent generation of reactive oxygen species by activated neutrophils and monocytes in unseparated human blood. Our data show that type II collagen-induced arthritis can be suppressed by the simultaneous inhibition of TNF-α, PARP, and NADPH oxidase.

Original languageEnglish
Pages (from-to)203-215
Number of pages13
JournalInflammation
Volume20
Issue number2
Publication statusPublished - 1996

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Thalidomide
Experimental Arthritis
Poly(ADP-ribose) Polymerases
Niacin
Amides
NADPH Oxidase
Arthritis
Luminescence
Monocytes
Anti-Idiotypic Antibodies
Reactive Oxygen Species
Neutrophils
Collagen
Poly(ADP-ribose) Polymerase Inhibitors

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Medicine(all)

Cite this

Synergistic effects of thalidomide and poly(ADP-ribose) polymerase inhibition on type II collagen-induced arthritis in mice. / Kröger, Hans; Miesel, Ralf; Dietrich, Annette; Ohde, Manuela; Rajnavolgyi, E.; Ockenfels, Heinrich.

In: Inflammation, Vol. 20, No. 2, 1996, p. 203-215.

Research output: Contribution to journalArticle

Kröger, Hans ; Miesel, Ralf ; Dietrich, Annette ; Ohde, Manuela ; Rajnavolgyi, E. ; Ockenfels, Heinrich. / Synergistic effects of thalidomide and poly(ADP-ribose) polymerase inhibition on type II collagen-induced arthritis in mice. In: Inflammation. 1996 ; Vol. 20, No. 2. pp. 203-215.
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