Synergistic effect of Avemar on proinflammatory cytokine production and Ras-mediated cell activation

András Telekes, Endre Kiss-Tóth, Tünde Nagy, Eva E. Qwarnstrom, Erzsébet Kúsz, Timea Polgár, Ákos Resetár, Steven K. Dower, Erno Duda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Macrophages activated by lipopolysaccharide and/or phorbol esters exhibited high sensitivity to Avemar, a fermented wheat germ extract. Avemar synergized with lipopolysaccharide and PMA in the induction of the transcription of cytokine genes and release of inflammatory cytokines. At higher concentrations the preparation had a significant negative effect on the proliferation and survival of activated myeloid cell types. Avemar treatment induced the synthesis of ICAM-1 and synergized with the ICAM-inducing effect of TNF, but had no effect on VCAM-1 expression on microvascular endotheilal cells. The effect of Avemar on signaling pathways, which are involved in cell activation was studied on HeLa cells as a model system. Avemar treatment increased the activity of stress kinases in a concentration-dependent way, resulting in the activation of AP-1 transcription factor. NF-kappa B-sensitive reporters were also activated by Avemar; in contrast, no effect of the preparation was observed on PKA-sensitive signaling pathways.

Original languageEnglish
Pages (from-to)515-528
Number of pages14
JournalAnnals of the New York Academy of Sciences
Volume1051
DOIs
Publication statusPublished - Jan 1 2005

Keywords

  • Avemar
  • Cytokine
  • ICAM
  • NF-κB
  • Synergism
  • TNF

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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