Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration

Alexey Tomilin, A. Reményi, Katharina Lins, Hanne Bak, Sebastian Leidel, Gerrit Vriend, Matthias Wilmanns, Hans R. Schöler

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer formed on the PORE (ATTTGAAATGCAAAT) can recruit the transcriptional coactivator OBF-1, whereas POU dimers formed on the consensus MORE (ATGCATATG CAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) fail to interact. An interaction with OBF-1 is precluded since the same Oct-1 residues that form the MORE dimerization interface are also used for OBF-1/Oct-1 interactions on the PORE. Our findings provide a paradigm of how specific POU dimer assemblies can differentially recruit a coregulatory activity with distinct transcriptional readouts.

Original languageEnglish
Pages (from-to)853-864
Number of pages12
JournalCell
Volume103
Issue number6
Publication statusPublished - 2000

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POU Domain Factors
Dimerization
Dimers
Immunoglobulin Heavy Chains
DNA
Monomers
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Tomilin, A., Reményi, A., Lins, K., Bak, H., Leidel, S., Vriend, G., ... Schöler, H. R. (2000). Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. Cell, 103(6), 853-864.

Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. / Tomilin, Alexey; Reményi, A.; Lins, Katharina; Bak, Hanne; Leidel, Sebastian; Vriend, Gerrit; Wilmanns, Matthias; Schöler, Hans R.

In: Cell, Vol. 103, No. 6, 2000, p. 853-864.

Research output: Contribution to journalArticle

Tomilin, A, Reményi, A, Lins, K, Bak, H, Leidel, S, Vriend, G, Wilmanns, M & Schöler, HR 2000, 'Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration', Cell, vol. 103, no. 6, pp. 853-864.
Tomilin, Alexey ; Reményi, A. ; Lins, Katharina ; Bak, Hanne ; Leidel, Sebastian ; Vriend, Gerrit ; Wilmanns, Matthias ; Schöler, Hans R. / Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. In: Cell. 2000 ; Vol. 103, No. 6. pp. 853-864.
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