Synchronization of medicago sativa cell suspension culture

Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, Gábor V. Horváth

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Deepening our knowledge on the regulation of the plant cell division cycle depends on techniques that allow for the enrichment of cell populations in defined cell cycle phases. Synchronization of cell division can be achieved using different plant tissues; however, well-established cell suspension cultures provide the largest amount of biological sample for further analysis. Here we describe the methodology of the establishment, propagation, and analysis of a Medicago sativa suspension culture that can be used for efficient synchronization of the cell division and also the application and removal of hydroxyurea blocking agent. A novel method is used for the estimation of cell portion that enters S phase during the assay. The protocol can be used in the case of other species as well.

Original languageEnglish
Title of host publicationCell Cycle Synchronization
Subtitle of host publicationMethods and Protocols
EditorsGaspar Banfalvi
Pages227-238
Number of pages12
DOIs
Publication statusPublished - Aug 4 2011

Publication series

NameMethods in Molecular Biology
Volume761
ISSN (Print)1064-3745

Keywords

  • 5-ethynyl-2′-deoxyuridine staining
  • Medicago sativa suspension culture
  • cell cycle synchronization
  • fluorescence microscopy
  • hydroxyurea

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Ayaydin, F., Kotogány, E., Ábrahám, E., & Horváth, G. V. (2011). Synchronization of medicago sativa cell suspension culture. In G. Banfalvi (Ed.), Cell Cycle Synchronization: Methods and Protocols (pp. 227-238). (Methods in Molecular Biology; Vol. 761). https://doi.org/10.1007/978-1-61779-182-6_15