Synchronization of Bacillus subtilis cells by spore germination and outgrowth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol defines conditions under which the germination of spores can be used to synchronize Bacillus subtilis cells, utilizing the time-ordered sequence of events taking place during the transition from spore to vegetative cells. The transition stages involve: phase change, swelling, emergence, initial division, and elongation. By using this method we have obtained two distinctive synchronized cell cycles, while the synchrony faded away in the third cycle. The advantage of using spore outgrowth and germination is that a highly synchronized population of bacterial cells can be obtained. Non-dividing spores stay synchronized, while synchrony rapidly decays during a few divisions. The limitations of this method are that it can be applied only for sporulating bacteria and synchrony lasts for only a limited period of time exceeding not more than two cycles.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages205-213
Number of pages9
Volume1524
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1524
ISSN (Print)10643745

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Keywords

  • DNA synthesis
  • Endospore formation
  • Permeabilization
  • Spectrophotometry
  • Spore outgrowth
  • Transition stage
  • Vegetative cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bánfalvi, G. (2017). Synchronization of Bacillus subtilis cells by spore germination and outgrowth. In Methods in Molecular Biology (Vol. 1524, pp. 205-213). (Methods in Molecular Biology; Vol. 1524). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6603-5_13