Synchronization of bacillus subtilis cells by spore germination and outgrowth

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

This protocol defines conditions under which the germination of spores can be used to synchronize Bacillus subtilis cells, utilizing the time-ordered sequence of events taking place during the transition from spore to vegetative cells. The transition stages involve: phase change, swelling, emergence, initial division, and elongation. By using this method we have obtained two distinctive synchronized cell cycles, while the synchrony faded away in the third cycle. The advantage of using spore outgrowth and germination is that a highly synchronized population of bacterial cells can be obtained. The limitations of this method are that it can be applied only for sporulating bacteria and synchrony lasts for only a limited period of time exceeding not more than two cycles.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages163-171
Number of pages9
Volume761
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
Volume761
ISSN (Print)10643745

Fingerprint

Germination
Bacillus subtilis
Spores
Cell Cycle
Bacteria
Population

Keywords

  • DNA synthesis
  • Endospore formation
  • permeabilization
  • spectrophotometry
  • spore outgrowth
  • transition stage
  • vegetative cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bánfalvi, G. (2011). Synchronization of bacillus subtilis cells by spore germination and outgrowth. In Methods in Molecular Biology (Vol. 761, pp. 163-171). (Methods in Molecular Biology; Vol. 761). https://doi.org/10.1007/978-1-61779-182-6_11

Synchronization of bacillus subtilis cells by spore germination and outgrowth. / Bánfalvi, G.

Methods in Molecular Biology. Vol. 761 2011. p. 163-171 (Methods in Molecular Biology; Vol. 761).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bánfalvi, G 2011, Synchronization of bacillus subtilis cells by spore germination and outgrowth. in Methods in Molecular Biology. vol. 761, Methods in Molecular Biology, vol. 761, pp. 163-171. https://doi.org/10.1007/978-1-61779-182-6_11
Bánfalvi G. Synchronization of bacillus subtilis cells by spore germination and outgrowth. In Methods in Molecular Biology. Vol. 761. 2011. p. 163-171. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-182-6_11
Bánfalvi, G. / Synchronization of bacillus subtilis cells by spore germination and outgrowth. Methods in Molecular Biology. Vol. 761 2011. pp. 163-171 (Methods in Molecular Biology).
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