Synchronization of bacillus subtilis cells by spore germination and outgrowth

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2 Citations (Scopus)

Abstract

This protocol defines conditions under which the germination of spores can be used to synchronize Bacillus subtilis cells, utilizing the time-ordered sequence of events taking place during the transition from spore to vegetative cells. The transition stages involve: phase change, swelling, emergence, initial division, and elongation. By using this method we have obtained two distinctive synchronized cell cycles, while the synchrony faded away in the third cycle. The advantage of using spore outgrowth and germination is that a highly synchronized population of bacterial cells can be obtained. The limitations of this method are that it can be applied only for sporulating bacteria and synchrony lasts for only a limited period of time exceeding not more than two cycles.

Original languageEnglish
Title of host publicationCell Cycle Synchronization
Subtitle of host publicationMethods and Protocols
EditorsGaspar Banfalvi
Pages163-171
Number of pages9
DOIs
Publication statusPublished - Aug 4 2011

Publication series

NameMethods in Molecular Biology
Volume761
ISSN (Print)1064-3745

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Keywords

  • DNA synthesis
  • Endospore formation
  • permeabilization
  • spectrophotometry
  • spore outgrowth
  • transition stage
  • vegetative cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Banfalvi, G. (2011). Synchronization of bacillus subtilis cells by spore germination and outgrowth. In G. Banfalvi (Ed.), Cell Cycle Synchronization: Methods and Protocols (pp. 163-171). (Methods in Molecular Biology; Vol. 761). https://doi.org/10.1007/978-1-61779-182-6_11