Synaptic proteome changes in the hypothalamus of mother rats

Edina Brigitta Udvari, Katalin Völgyi, Péter Gulyássy, Diána Dimén, Viktor Kis, János Barna, Éva Rebeka Szabó, Gert Lubec, G. Juhász, K. Kékesi, A. Dobolyi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To establish synaptic proteome changes associated with motherhood, we isolated synaptosome fractions from the hypothalamus of mother rats and non-maternal control females at the 11th postpartum day. Proteomic analysis by two-dimensional differential gel electrophoresis combined with mass spectrometric protein identification established 26 significant proteins, 7 increasing and 19 decreasing protein levels in the dams. The altered proteins are mainly involved in energy homeostasis, protein folding, and metabolic processes suggesting the involvement of these cellular processes in maternal adaptations. The decrease in a significantly altered protein, complement component 1q subcomponent-binding protein (C1qbp) was validated with Western blotting. Furthermore, immunohistochemistry showed its presence in hypothalamic fibers and terminals in agreement with its presence in synaptosomes. We also found the expression of C1qbp in different hypothalamic nuclei including the preoptic area and the paraventricular hypothalamic nucleus at the protein and at the mRNA level using immunohistochemistry and in situ hybridization histochemistry, respectively. Bioinformatical network analysis revealed that cytokines, growth factors, and protein kinases are common regulators, which indicates a complex regulation of the proteome change in mothers. The results suggest that maternal responsiveness is associated with synaptic proteins level changes in the hypothalamus, and that growth factors and cytokines may govern these alterations. Biological significance The period of motherhood is accompanied with several behavioral, neuroendocrine, emotional and metabolic adaptations in the brain. Although it is established that various hypothalamic networks participate in the maternal adaptations of the rodent brain, our knowledge on the molecular background of these alterations remains seriously limited. In the present study, we first determined that the functional alterations of the maternal brain can be detected at the level of the synaptic proteome in the hypothalamus. Independent confirmation of synaptic localization, and also the established decrease in the level of C1qbp protein suggest the validity of the data. Common regulators of altered proteins belonging to the growth factor and cytokine family suggest that the synaptic adaptation is governed by these extracellular signals and future studies should focus on their specific roles. Our study was also the first to describe the expression pattern of C1qbp in the hypothalamus, a protein potentially involved in mitochondrial and neuroimmunological regulations of synaptic plasticity. Its presence in the preoptic area responsible for maternal behaviors and also in the paraventricular hypothalamic and arcuate nuclei regulating hormonal levels suggests that the same proteins may be involved in different aspects of maternal adaptations. The conclusions of the present work contribute to establishing the molecular alterations that determine different maternal adaptations in the brain. Since maternal changes are models of neuronal plasticity in all social interactions, the reported results can affect a wide field of molecular and behavioral neuroscience.

Original languageEnglish
Pages (from-to)54-66
Number of pages13
JournalJournal of Proteomics
Volume159
DOIs
Publication statusPublished - Apr 21 2017

Fingerprint

Proteome
Hypothalamus
Rats
Mothers
Proteins
Brain
Carrier Proteins
Intercellular Signaling Peptides and Proteins
Neuronal Plasticity
Preoptic Area
Synaptosomes
Paraventricular Hypothalamic Nucleus
Cytokines
Plasticity
Complement C1q
Immunohistochemistry
Protein folding
Maternal Behavior
Arcuate Nucleus of Hypothalamus
Electric network analysis

Keywords

  • 2-D DIGE
  • C1qbp
  • Common regulator
  • Lactation
  • Maternal behavior
  • Synaptosome

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Udvari, E. B., Völgyi, K., Gulyássy, P., Dimén, D., Kis, V., Barna, J., ... Dobolyi, A. (2017). Synaptic proteome changes in the hypothalamus of mother rats. Journal of Proteomics, 159, 54-66. https://doi.org/10.1016/j.jprot.2017.03.006

Synaptic proteome changes in the hypothalamus of mother rats. / Udvari, Edina Brigitta; Völgyi, Katalin; Gulyássy, Péter; Dimén, Diána; Kis, Viktor; Barna, János; Szabó, Éva Rebeka; Lubec, Gert; Juhász, G.; Kékesi, K.; Dobolyi, A.

In: Journal of Proteomics, Vol. 159, 21.04.2017, p. 54-66.

Research output: Contribution to journalArticle

Udvari, EB, Völgyi, K, Gulyássy, P, Dimén, D, Kis, V, Barna, J, Szabó, ÉR, Lubec, G, Juhász, G, Kékesi, K & Dobolyi, A 2017, 'Synaptic proteome changes in the hypothalamus of mother rats', Journal of Proteomics, vol. 159, pp. 54-66. https://doi.org/10.1016/j.jprot.2017.03.006
Udvari EB, Völgyi K, Gulyássy P, Dimén D, Kis V, Barna J et al. Synaptic proteome changes in the hypothalamus of mother rats. Journal of Proteomics. 2017 Apr 21;159:54-66. https://doi.org/10.1016/j.jprot.2017.03.006
Udvari, Edina Brigitta ; Völgyi, Katalin ; Gulyássy, Péter ; Dimén, Diána ; Kis, Viktor ; Barna, János ; Szabó, Éva Rebeka ; Lubec, Gert ; Juhász, G. ; Kékesi, K. ; Dobolyi, A. / Synaptic proteome changes in the hypothalamus of mother rats. In: Journal of Proteomics. 2017 ; Vol. 159. pp. 54-66.
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AU - Kis, Viktor

AU - Barna, János

AU - Szabó, Éva Rebeka

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