Synaptic contacts of tyrosine hydroxylase-immunoreactive elements in the inner plexiform layer of the retina of Bufo marinus

R. Gábriel, Baosong Zhu, Charles Straznicky

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tyrosine hydroxylase (TH) immunocytochemistry was utilized to quantify dopaminergic synapses in the inner plexiform layer of the retina of Bufo marinus. Since dopaminergic cells have bistratified dendritic arborisation in the inner plexiform layer, attention was given to the segregation of synapses between the scleral and the vitreal sublaminae. Light-microscopically, a more elaborate dendritic branching was observed in the scleral than in the vitreal sublamina. In contrast, about 55% of synapses occurred in the vitreal one fifth of the inner plexiform layer, 30% in the scleral fifth, and 15% in the intermediate laminae. Input sources and output targets showed only minor quantitative differences between sublaminae 1 and 5. TH-immunoreactive processes were found in presynaptic (62.8%) and postsynaptic (37.2%) positions. Synapses to the stained dendrites derived from bipolar (40.4%) and amacrine (59.6%) cells, whereas outputs from the TH-positive processes were directed to amacrine cells (56.8%) and to small and medium-sized dendrites (35.4%); at least some of these can be considered as ganglion cell dendrites. TH-positive profiles neither formed synapses with each other nor were presynaptic to bipolar cell terminals. Junctional appositions of the immunoreactive profiles were occasionally seen on non-stained amacrine and ganglion cell dendrites in the scleral sublamina of the inner plexiform layer and on optic axons in the optic fibre layer. Although dopaminergic cells are mainly involved in amacrine-amacrine interactions, inputs from bipolar terminals and outputs to ganglion cell dendrites were also substantial, suggestive of a role also in vertical information processing.

Original languageEnglish
Pages (from-to)525-534
Number of pages10
JournalCell and Tissue Research
Volume267
Issue number3
DOIs
Publication statusPublished - Mar 1992

Fingerprint

Bufo marinus
Tyrosine 3-Monooxygenase
Dendrites
Synapses
Retina
Amacrine Cells
Ganglia
Neuronal Plasticity
Fiber optics
Optics
Automatic Data Processing
Axons
Immunohistochemistry
Light

Keywords

  • Bufo marinus (Anura)
  • Dopaminergic neurons
  • Electron microscopy
  • Immunocytochemistry
  • Inner plexiform layer
  • Retina
  • Synapses

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Synaptic contacts of tyrosine hydroxylase-immunoreactive elements in the inner plexiform layer of the retina of Bufo marinus. / Gábriel, R.; Zhu, Baosong; Straznicky, Charles.

In: Cell and Tissue Research, Vol. 267, No. 3, 03.1992, p. 525-534.

Research output: Contribution to journalArticle

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