Syk is required for integrin signaling in neutrophils

A. Mócsai, Meijuan Zhou, Fanying Meng, Victor L. Tybulewicz, Clifford A. Lowell

Research output: Contribution to journalArticle

300 Citations (Scopus)

Abstract

The Syk tyrosine kinase plays a critical role in the signaling machinery of various receptors of the adaptive immune system. Here we show that Syk is also an essential component of integrin signaling in neutrophils. syk-/- neutrophils failed to undergo respiratory burst, degranulation, or spreading in response to proinflammatory stimuli while adherent to immobilized integrin ligands or when stimulated by direct crosslinking of integrins. Signaling from the β1, β2, or β3 integrins was defective in syk-/- cells. Syk colocalized with CD18 during cell spreading and initiated downstream signaling events leading to actin polymerization. Surprisingly, these defects in integrin-mediated activation did not impair the integrin-dependent in vitro or in vivo migration of syk-/- neutrophils or of cells deficient in Src-family kinases. Thus, integrins use different signaling mechanisms to support migration and adherent activation.

Original languageEnglish
Pages (from-to)547-558
Number of pages12
JournalImmunity
Volume16
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Integrins
Neutrophils
Respiratory Burst
src-Family Kinases
Polymerization
Protein-Tyrosine Kinases
Actins
Immune System
Ligands

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Mócsai, A., Zhou, M., Meng, F., Tybulewicz, V. L., & Lowell, C. A. (2002). Syk is required for integrin signaling in neutrophils. Immunity, 16(4), 547-558. https://doi.org/10.1016/S1074-7613(02)00303-5

Syk is required for integrin signaling in neutrophils. / Mócsai, A.; Zhou, Meijuan; Meng, Fanying; Tybulewicz, Victor L.; Lowell, Clifford A.

In: Immunity, Vol. 16, No. 4, 2002, p. 547-558.

Research output: Contribution to journalArticle

Mócsai, A, Zhou, M, Meng, F, Tybulewicz, VL & Lowell, CA 2002, 'Syk is required for integrin signaling in neutrophils', Immunity, vol. 16, no. 4, pp. 547-558. https://doi.org/10.1016/S1074-7613(02)00303-5
Mócsai, A. ; Zhou, Meijuan ; Meng, Fanying ; Tybulewicz, Victor L. ; Lowell, Clifford A. / Syk is required for integrin signaling in neutrophils. In: Immunity. 2002 ; Vol. 16, No. 4. pp. 547-558.
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