Switch movements and the myosin crossbridge stroke

A. Málnási-Csizmadia, Jane L. Dickens, Wei Zeng, Clive R. Bagshaw

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The myosin II motor from Dictyostelium discoideum has been engineered to contain single tryptophan residues at strategic locations to probe movements of switch 1 and switch 2. The tryptophan residue at W501 probes movement of the relay helix and indirectly reports on switch 2 movement. This probe suggests that there is an equilibrium between the switch 2 open- and closed-states when the γ-phosphate position is occupied. Actin does not appear to greatly affect this equilibrium directly, but has indirect influence via switch 1. The latter region has been probed by introducing tryptophan residues at positions 239 and 242. The kinetics of the actomyosin ATPase in solution is discussed with respect to recent crossbridge models based on high-resolution crystal structures.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalJournal of Muscle Research and Cell Motility
Volume26
Issue number1
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Myosins
Tryptophan
Stroke
Switches
Myosin Type II
Dictyostelium
Actins
Phosphates
Crystal structure
Kinetics

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Switch movements and the myosin crossbridge stroke. / Málnási-Csizmadia, A.; Dickens, Jane L.; Zeng, Wei; Bagshaw, Clive R.

In: Journal of Muscle Research and Cell Motility, Vol. 26, No. 1, 02.2005, p. 31-37.

Research output: Contribution to journalArticle

Málnási-Csizmadia, A. ; Dickens, Jane L. ; Zeng, Wei ; Bagshaw, Clive R. / Switch movements and the myosin crossbridge stroke. In: Journal of Muscle Research and Cell Motility. 2005 ; Vol. 26, No. 1. pp. 31-37.
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