Switch from ER-mitochondrial to SR-mitochondrial calcium coupling during muscle differentiation

Muqing Yi, David Weaver, Verónica Eisner, P. Várnai, L. Hunyady, Jianjie Ma, György Csordás, György Hajnóczky

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Emerging evidence indicates that mitochondria are locally coupled to endoplasmic reticulum (ER) Ca2+ release in myoblasts and to sarcoplasmic reticulum (SR) Ca2+ release in differentiated muscle fibers in order to regulate cytoplasmic calcium dynamics and match metabolism with cell activity. However, the mechanism of the developmental transition from ER to SR coupling remains unclear. We have studied mitochondrial sensing of IP3 receptor (IP3R)- and ryanodine receptor (RyR)-mediated Ca2+ signals in H9c2 myoblasts and differentiating myotubes, as well as the attendant changes in mitochondrial morphology. Mitochondria in myoblasts were largely elongated, luminally connected and relatively few in number, whereas the myotubes were densely packed with globular mitochondria that displayed limited luminal continuity. Vasopressin, an IP3-linked agonist, evoked a large cytoplasmic Ca2+ ([Ca2+]c) increase in myoblasts, whereas it elicited a smaller response in myotubes. Conversely, RyR-mediated Ca2+ release induced by caffeine, was not observed in myoblasts, but triggered a large [Ca2+]c signal in myotubes. Both the IP3R and the RyR-mediated [Ca2+]c rise was closely associated with a mitochondrial matrix Ca2+ ([Ca2+]m) signal. Every myotube that showed a [Ca2+]c spike also displayed a [Ca2+]m response. Addition of IP3 to permeabilized myoblasts and caffeine to permeabilized myotubes also resulted in a rapid [Ca2+]m rise, indicating that Ca2+ was delivered via local coupling of the ER/SR and mitochondria. Thus, as RyRs are expressed during muscle differentiation, the local connection between RyR and mitochondrial Ca2+ uptake sites also appears. When RyR1 was exogenously introduced to myoblasts by overexpression, the [Ca2+]m signal appeared together with the [Ca2+]c signal, however the mitochondrial morphology remained unchanged. Thus, RyR expression alone is sufficient to induce the steps essential for their alignment with mitochondrial Ca2+ uptake sites, whereas the mitochondrial proliferation and reshaping utilize either downstream or alternative pathways.

Original languageEnglish
Pages (from-to)355-365
Number of pages11
JournalCell Calcium
Volume52
Issue number5
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Myoblasts
Sarcoplasmic Reticulum
Ryanodine Receptor Calcium Release Channel
Skeletal Muscle Fibers
Endoplasmic Reticulum
Calcium
Muscles
Mitochondria
Inositol 1,4,5-Trisphosphate Receptors
Caffeine
Vasopressins

Keywords

  • Calcium signaling
  • Development
  • Mitochondria
  • Mitochondria-associated membranes (Mam)
  • Ryanodine receptor
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Switch from ER-mitochondrial to SR-mitochondrial calcium coupling during muscle differentiation. / Yi, Muqing; Weaver, David; Eisner, Verónica; Várnai, P.; Hunyady, L.; Ma, Jianjie; Csordás, György; Hajnóczky, György.

In: Cell Calcium, Vol. 52, No. 5, 11.2012, p. 355-365.

Research output: Contribution to journalArticle

Yi, Muqing ; Weaver, David ; Eisner, Verónica ; Várnai, P. ; Hunyady, L. ; Ma, Jianjie ; Csordás, György ; Hajnóczky, György. / Switch from ER-mitochondrial to SR-mitochondrial calcium coupling during muscle differentiation. In: Cell Calcium. 2012 ; Vol. 52, No. 5. pp. 355-365.
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