Survival of mouse blastocysts after low-temperature preservation under high pressure

Cs Pribenszky, M. Molnár, S. Cseh, L. Solti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.

Original languageEnglish
Pages (from-to)479-487
Number of pages9
JournalActa Veterinaria Hungarica
Volume52
Issue number4
DOIs
Publication statusPublished - 2004

Fingerprint

Blastocyst
blastocyst
embryo (animal)
Embryonic Structures
Pressure
Temperature
mice
Hydrostatic Pressure
temperature
Freezing
Decompression
freezing
Transition Temperature
Phase Transition
Cryopreservation
freezing point
phase transition
cryopreservation
preserves
ambient temperature

Keywords

  • Blastocyst
  • Embryo cryopreservation
  • Freezing
  • Freezing point depression
  • High hydrostatic pressure

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Survival of mouse blastocysts after low-temperature preservation under high pressure. / Pribenszky, Cs; Molnár, M.; Cseh, S.; Solti, L.

In: Acta Veterinaria Hungarica, Vol. 52, No. 4, 2004, p. 479-487.

Research output: Contribution to journalArticle

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