Survival and proliferative ability of various living cell types after laser-induced forward transfer

Béla Hopp, Tomi Smausz, Norbert Kresz, Norbert Barna, Zsolt Bor, Lajos Kolozsvári, Douglas B. Chrisey, András Szabó, Antal Nógrádi

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193 Citations (Scopus)

Abstract

The survival, proliferation, and differentiation of freshly isolated and cultured cells were studied after absorbing film-assisted laser-induced forward transfer. Rat Schwann and astroglial cells and pig lens epithelial cells were used for transfer and the cells were cultured for 2 weeks after laser-pulsed transfer. All three cell types survived, proliferated, and differentiated under cell culture conditions and regained their original phenotype a few days after cell transfer. Time resolution studies have shown that the time required to accelerate the jets and droplets containing the cells was less than 1 μs and that the estimated minimum average acceleration of those ejected cells that reached a constant velocity was approximately 107 × g. This suggests that the majority of studied cells tolerated the extremely high acceleration at the beginning of the ejection and the deceleration during impact on the acceptor plate without significant damage to the original phenotype. These results suggest that the absorbing film-assisted laser-induced forward transfer technique appears to be suitable for several potential applications in tissue engineering and the biomedical tissue repair technologies.

Original languageEnglish
Pages (from-to)1817-1823
Number of pages7
JournalTissue Engineering
Volume11
Issue number11-12
DOIs
Publication statusPublished - Nov 1 2005

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ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Cell Biology

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