Survey of the aflatoxin gene cluster in Aspergilli from hungarian crops

Szilvia Kovács, T. Pusztahelyi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Climate changes in Europe, which is characterized by the decrease of rainy days and the higher average temperature at summer, significantly increase the spreading of Aspergillus species and aflatoxin B1 contamination of the staple food and feed materials. The aim of our study was to estimate the possibility of the aflatoxin production of the Aspergilli on crops. From the isolates that were gained from crop samples, higher than 40% of the Aspergillus isolates contained norA, aflR and omtA genes from the aflatoxin genes cluster. Most of these isolates (63%) showed high homology with A. flavus, while three isolates showed high homology to A. tritici/A. candidus, one to A. cristatus/A. amstelodami and one strain showed the highest homology to A. tritici. Six from the A. flavus isolates (85.7%) with norA, aflR and omtA genes could produce aflatoxin B1 on malate extract agar medium. Parallel PCR and toxin measurements are recommended to evaluate the potentiality of aflatoxin production.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalActa Phytopathologica et Entomologica Hungarica
Volume52
Issue number2
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

multigene family
Aspergillus
aflatoxins
aflatoxin B1
crops
staple foods
malates
Aspergillus flavus
toxins
genes
agar
climate change
summer
extracts
temperature
sampling

Keywords

  • Aflatoxin B1
  • Aspergillus flavus
  • ITS
  • PCR

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

Cite this

Survey of the aflatoxin gene cluster in Aspergilli from hungarian crops. / Kovács, Szilvia; Pusztahelyi, T.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 52, No. 2, 01.12.2017, p. 169-176.

Research output: Contribution to journalArticle

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