Survey of babesiosis in symptomatic dogs from Romania: Occurrence of Babesia gibsoni associated with breed

Mirela Imre, R. Farkas, Marius Stelian Ilie, Kálmán Imre, Gheorghe Dǎrǎbuş

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Blood samples from 49 symptomatic dogs from 5 western and north-western counties of Romania were screened using microscopic examination, polymerase-chain-reaction-restriction-fragment-length-polymorphism procedure (PCR-RFLP), and sequence analysis. Results of the microscopic evaluation of stained blood smears revealed 45 positive samples with the presence of large and small intraerythrocytic piroplasms in 35 and 10 samples, respectively. Babesia canis (35/49, 71.4%) and Babesia gibsoni (14/49, 28.6%) were identified and differentiated by PCR-RFLP targeting the 18S rRNA gene. Results of the sequence analysis of all B. gibsoni and 17 randomly selected B. canis PCR products confirmed the PCR-RFLP-diagnosed species. The distribution of B. gibsoni infection was positively associated (p

Original languageEnglish
Pages (from-to)500-502
Number of pages3
JournalTicks and Tick-borne Diseases
Volume4
Issue number6
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Babesia gibsoni
Babesiosis
Babesia
Romania
babesiosis
Babesia canis
restriction fragment length polymorphism
polymerase chain reaction
Restriction Fragment Length Polymorphisms
Dogs
breeds
Polymerase Chain Reaction
dogs
sequence analysis
Sequence Analysis
Piroplasmida
blood
sampling
rRNA Genes
ribosomal RNA

Keywords

  • Babesia canis
  • Babesia gibsoni
  • Babesiosis
  • Dog
  • Fighting breed
  • Romania

ASJC Scopus subject areas

  • Infectious Diseases
  • Insect Science
  • Parasitology
  • Microbiology
  • Medicine(all)

Cite this

Survey of babesiosis in symptomatic dogs from Romania : Occurrence of Babesia gibsoni associated with breed. / Imre, Mirela; Farkas, R.; Ilie, Marius Stelian; Imre, Kálmán; Dǎrǎbuş, Gheorghe.

In: Ticks and Tick-borne Diseases, Vol. 4, No. 6, 12.2013, p. 500-502.

Research output: Contribution to journalArticle

Imre, Mirela ; Farkas, R. ; Ilie, Marius Stelian ; Imre, Kálmán ; Dǎrǎbuş, Gheorghe. / Survey of babesiosis in symptomatic dogs from Romania : Occurrence of Babesia gibsoni associated with breed. In: Ticks and Tick-borne Diseases. 2013 ; Vol. 4, No. 6. pp. 500-502.
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