Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains

Brigitta László, József Kónya, Eszter Dandár, Judit Deák, Ágnes Farkas, Jim Gray, Gábor Grósz, Miren Iturriza-Gomara, Ferenc Jakab, Ágnes Juhász, Péter Kisfali, Julianna Kovács, György Lengyel, Vito Martella, Béla Melegh, Júlia Mészáros, Péter Molnár, Zoltán Nyúl, Hajnalka Papp, László PátriErzsébet Puskás, Ildikó Sántha, Ferenc Schneider, Katalin Szomor, András Tóth, Erzsébet Tóth, György Szuács, Krisztián Bányai

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Abstract

Background: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. Objectives: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. Study design: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5. years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. Results: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. Conclusions: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.

Original languageEnglish
Pages (from-to)140-146
Number of pages7
JournalJournal of Clinical Virology
Volume55
Issue number2
DOIs
Publication statusPublished - Oct 1 2012

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Keywords

  • Gastroenteritis
  • Genotype
  • Molecular epidemiology
  • VP4
  • VP7

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

László, B., Kónya, J., Dandár, E., Deák, J., Farkas, Á., Gray, J., Grósz, G., Iturriza-Gomara, M., Jakab, F., Juhász, Á., Kisfali, P., Kovács, J., Lengyel, G., Martella, V., Melegh, B., Mészáros, J., Molnár, P., Nyúl, Z., Papp, H., ... Bányai, K. (2012). Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains. Journal of Clinical Virology, 55(2), 140-146. https://doi.org/10.1016/j.jcv.2012.06.016