Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

Badri L. Aekbote, Tamás Fekete, Jaroslaw Jacak, Gaszton Vizsnyiczai, P. Ormos, L. Kelemen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and nonspecifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation.

Original languageEnglish
Pages (from-to)45-56
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 7 2015

Fingerprint

manipulators
Optical Tweezers
microstructure
Streptavidin
cells
Photons
Polymerization
polymerization
trapping
Micromanipulation
proteins
Diamines
photons
diamines
antibodies
actuation
coating
glycols
polyethylenes
Proteins

Keywords

  • Biomaterials
  • Fluorescence microscopy
  • Laser materials processing
  • Microstructure fabrication
  • Optical tweezers or optical manipulation
  • Polymers
  • Spatial light modulators
  • Thin films
  • Ultrafast lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells. / Aekbote, Badri L.; Fekete, Tamás; Jacak, Jaroslaw; Vizsnyiczai, Gaszton; Ormos, P.; Kelemen, L.

In: Biomedical Optics Express, Vol. 7, No. 1, 07.12.2015, p. 45-56.

Research output: Contribution to journalArticle

Aekbote, Badri L. ; Fekete, Tamás ; Jacak, Jaroslaw ; Vizsnyiczai, Gaszton ; Ormos, P. ; Kelemen, L. / Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells. In: Biomedical Optics Express. 2015 ; Vol. 7, No. 1. pp. 45-56.
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