Surface modification of HSA containing magnetic PLGA nanoparticles by poloxamer to decrease plasma protein adsorption

Quazi T.H. Shubhra, Judit Tóth, János Gyenis, Tivadar Feczkó

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Lifetime prolongation for hydrophobic drug carriers has been the focus of interest for many years. Poloxamer (Pluronic F68, PF68) has been employed in this study for modifying the surface of magnetic poly(d,. l-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with human serum albumin (HSA) model drug. Surface characteristics of untreated and PF68 treated NPs were analyzed by size, zeta potential and electrophoretic mobility studies. UV-vis spectroscopic analysis, isothermal titration calorimetry (ITC) and dynamic light scattering methods were used to investigate serum protein (bovine serum albumin, BSA) adsorption. Results showed the successful surface attachment of PF68. Among different concentrations (0.1-1%, wt/vol) of PF68 studied, 0.5% was found to be the most useful, since a higher concentration can issue in micelle formation. 50% less BSA tended to be adsorbed on the treated NPs in comparison to the untreated ones.

Original languageEnglish
Pages (from-to)529-536
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume122
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Isothermal titration calorimetry
  • Poloxamer
  • Protein adsorption
  • Surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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