Surface layer modification of PLGA nanoparticles with targeting peptide

A convenient synthetic route for Pluronic F127 - Tuftsin conjugate

Kata Horváti, Gergo Gyulai, A. Csámpai, János Rohonczy, E. Kiss, Sz. Bősze

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nanoparticles consist of biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) are promising carriers for drug molecules to improve the treatment of tuberculosis. Surface modifiers, such as Pluronic F127, are essential for biocompatibility and for the protection against particle aggregation. This study demonstrates a successful approach to conjugate Pluronic F127 coated PLGA nanoparticles with Tuftsin, which has been reported as a macrophage-targeting peptide. Transformation of Pluronic F127 hydroxyl groups - which have limited reactivity - into aldehyde groups provide a convenient way to bind aminooxy-peptide derivatives in one step reaction. We have also investigated that this change has no effect on the physico-chemical properties of the nanoparticles. Our data showed that coating nanoparticles with Pluronic-Tuftsin conjugate markedly increased the internalization rate and the intracellular activity of the encapsulated drug candidate against Mycobacterium tuberculosis. Employing this approach, a large variety of peptide targeted PLGA nanoparticles can be designed for drug delivery.

Original languageEnglish
JournalUnknown Journal
DOIs
Publication statusAccepted/In press - Mar 2 2018

Fingerprint

UCON 50-HB-5100
Tuftsin
Poloxamer
Nanoparticles
Peptides
Drug Carriers
Macrophages
Aldehydes
Drug delivery
Biocompatibility
Mycobacterium tuberculosis
Pharmaceutical Preparations
Hydroxyl Radical
Chemical properties
Tuberculosis
Agglomeration
polylactic acid-polyglycolic acid copolymer
peptide A
Derivatives
Coatings

Keywords

  • macrophage targeting, Tuftsin
  • PLGA nanoparticles
  • Pluronic labeling

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

@article{dd47114bd0c54c5dba1a6c30372a26b4,
title = "Surface layer modification of PLGA nanoparticles with targeting peptide: A convenient synthetic route for Pluronic F127 - Tuftsin conjugate",
abstract = "Nanoparticles consist of biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) are promising carriers for drug molecules to improve the treatment of tuberculosis. Surface modifiers, such as Pluronic F127, are essential for biocompatibility and for the protection against particle aggregation. This study demonstrates a successful approach to conjugate Pluronic F127 coated PLGA nanoparticles with Tuftsin, which has been reported as a macrophage-targeting peptide. Transformation of Pluronic F127 hydroxyl groups - which have limited reactivity - into aldehyde groups provide a convenient way to bind aminooxy-peptide derivatives in one step reaction. We have also investigated that this change has no effect on the physico-chemical properties of the nanoparticles. Our data showed that coating nanoparticles with Pluronic-Tuftsin conjugate markedly increased the internalization rate and the intracellular activity of the encapsulated drug candidate against Mycobacterium tuberculosis. Employing this approach, a large variety of peptide targeted PLGA nanoparticles can be designed for drug delivery.",
keywords = "macrophage targeting, Tuftsin, PLGA nanoparticles, Pluronic labeling",
author = "Kata Horv{\'a}ti and Gergo Gyulai and A. Cs{\'a}mpai and J{\'a}nos Rohonczy and E. Kiss and Sz. Bősze",
year = "2018",
month = "3",
day = "2",
doi = "10.1021/acs.bioconjchem.8b00156",
language = "English",
journal = "[No source information available]",
issn = "0402-1215",

}

TY - JOUR

T1 - Surface layer modification of PLGA nanoparticles with targeting peptide

T2 - A convenient synthetic route for Pluronic F127 - Tuftsin conjugate

AU - Horváti, Kata

AU - Gyulai, Gergo

AU - Csámpai, A.

AU - Rohonczy, János

AU - Kiss, E.

AU - Bősze, Sz.

PY - 2018/3/2

Y1 - 2018/3/2

N2 - Nanoparticles consist of biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) are promising carriers for drug molecules to improve the treatment of tuberculosis. Surface modifiers, such as Pluronic F127, are essential for biocompatibility and for the protection against particle aggregation. This study demonstrates a successful approach to conjugate Pluronic F127 coated PLGA nanoparticles with Tuftsin, which has been reported as a macrophage-targeting peptide. Transformation of Pluronic F127 hydroxyl groups - which have limited reactivity - into aldehyde groups provide a convenient way to bind aminooxy-peptide derivatives in one step reaction. We have also investigated that this change has no effect on the physico-chemical properties of the nanoparticles. Our data showed that coating nanoparticles with Pluronic-Tuftsin conjugate markedly increased the internalization rate and the intracellular activity of the encapsulated drug candidate against Mycobacterium tuberculosis. Employing this approach, a large variety of peptide targeted PLGA nanoparticles can be designed for drug delivery.

AB - Nanoparticles consist of biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) are promising carriers for drug molecules to improve the treatment of tuberculosis. Surface modifiers, such as Pluronic F127, are essential for biocompatibility and for the protection against particle aggregation. This study demonstrates a successful approach to conjugate Pluronic F127 coated PLGA nanoparticles with Tuftsin, which has been reported as a macrophage-targeting peptide. Transformation of Pluronic F127 hydroxyl groups - which have limited reactivity - into aldehyde groups provide a convenient way to bind aminooxy-peptide derivatives in one step reaction. We have also investigated that this change has no effect on the physico-chemical properties of the nanoparticles. Our data showed that coating nanoparticles with Pluronic-Tuftsin conjugate markedly increased the internalization rate and the intracellular activity of the encapsulated drug candidate against Mycobacterium tuberculosis. Employing this approach, a large variety of peptide targeted PLGA nanoparticles can be designed for drug delivery.

KW - macrophage targeting, Tuftsin

KW - PLGA nanoparticles

KW - Pluronic labeling

UR - http://www.scopus.com/inward/record.url?scp=85046484412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046484412&partnerID=8YFLogxK

U2 - 10.1021/acs.bioconjchem.8b00156

DO - 10.1021/acs.bioconjchem.8b00156

M3 - Article

JO - [No source information available]

JF - [No source information available]

SN - 0402-1215

ER -