Surface coatings shape the protein corona of SPIONs with relevance to their application in vivo

Angéla Jedlovszky-Hajdú, Francesca Baldelli Bombelli, Marco P. Monopoli, Etelka Tombácz, Kenneth A. Dawson

Research output: Contribution to journalArticle

98 Citations (Scopus)


Superparamagnetic iron oxide nanoparticles (SPIONs) have proved their use in many biomedical applications, such as drug delivery, hyperthermia, and MRI (magnetic resonance imaging) contrast agents. Due to their instability in fluids, several surface coatings have been used to both stabilize and tune the properties of these nanoparticles (NPs) according to their applications. These coatings will strongly modify their surface properties and influence their interaction with the environment proteins in a relevant biological medium with a clear impact on their function. It is well-accepted that a protein corona is immediately formed when nanoparticles come in contact with a biological milieu, and the emergent bionano interface represents the biological identity of the particles. Here, we investigate how a different coating on the same magnetic core can influence the protein corona composition and structure with clear relevance to application of these NPs in medicine. In particular, we have studied the structure and composition of the protein corona-SPION complexes of magnetite nanoparticles stabilized with citric acid, poly(acrylic acid), or double layer oleic acid by a range of approaches, including dynamic light scattering, nanoparticle tracking analysis, differential centrifugal sedimentation, infrared spectroscopy, 1-D SDS gel electrophoresis, and mass spectroscopy.

Original languageEnglish
Pages (from-to)14983-14991
Number of pages9
Issue number42
Publication statusPublished - Oct 23 2012


ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Jedlovszky-Hajdú, A., Bombelli, F. B., Monopoli, M. P., Tombácz, E., & Dawson, K. A. (2012). Surface coatings shape the protein corona of SPIONs with relevance to their application in vivo. Langmuir, 28(42), 14983-14991.