Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells

Gaspar Banfalvi, Gyorgy Trencsenyi, Kinga Ujvarosi, Gabor Nagy, Timea Ombodi, Monika Bedei, Csilla Somogyi, Alexei G. Basnakian

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Earlier, the interphase chromatin structures could not be visualized due to the stickiness of the nuclear material. We have reduced stickiness by the reversal of permeabilization allowing the isolation and microscopic imaging of interphase chromatin structures. By using a high resolution of synchronization, collecting 36 elutriation fractions, we show that major intermediates of chromatin condensation include: (a) decondensed veil-like chromatin at the unset of the S phase (2.0-2.2 C-value), (b) polarization of veiled chromatin (2.2-2.6 C), (c) fibrous chromatin (2.6-3.0 C), chromatin bodies (3.0-3.3 C), early precondensed chromosomes (3.3-3.6). The compaction of Drosophila chromosomes did not reach that of the mammalian cells in the final stage of condensation (3.6-4.0 C). Drosophila chromosomes consist of smaller units called rodlets. Results demonstrate that nucleosomal chromatin ("beads on string") does not form a solenoid structure; rather, the topological arrangement consists of meandering and plectonemic loops.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalDNA and Cell Biology
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Drosophila
Chromatin
Chromosomes
Interphase
S Phase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Banfalvi, G., Trencsenyi, G., Ujvarosi, K., Nagy, G., Ombodi, T., Bedei, M., ... Basnakian, A. G. (2007). Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells. DNA and Cell Biology, 26(1), 55-62. https://doi.org/10.1089/dna.2006.0524

Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells. / Banfalvi, Gaspar; Trencsenyi, Gyorgy; Ujvarosi, Kinga; Nagy, Gabor; Ombodi, Timea; Bedei, Monika; Somogyi, Csilla; Basnakian, Alexei G.

In: DNA and Cell Biology, Vol. 26, No. 1, 01.2007, p. 55-62.

Research output: Contribution to journalArticle

Banfalvi, G, Trencsenyi, G, Ujvarosi, K, Nagy, G, Ombodi, T, Bedei, M, Somogyi, C & Basnakian, AG 2007, 'Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells', DNA and Cell Biology, vol. 26, no. 1, pp. 55-62. https://doi.org/10.1089/dna.2006.0524
Banfalvi, Gaspar ; Trencsenyi, Gyorgy ; Ujvarosi, Kinga ; Nagy, Gabor ; Ombodi, Timea ; Bedei, Monika ; Somogyi, Csilla ; Basnakian, Alexei G. / Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells. In: DNA and Cell Biology. 2007 ; Vol. 26, No. 1. pp. 55-62.
@article{9ee8e344690749c3a38b1a820e1ada01,
title = "Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells",
abstract = "Earlier, the interphase chromatin structures could not be visualized due to the stickiness of the nuclear material. We have reduced stickiness by the reversal of permeabilization allowing the isolation and microscopic imaging of interphase chromatin structures. By using a high resolution of synchronization, collecting 36 elutriation fractions, we show that major intermediates of chromatin condensation include: (a) decondensed veil-like chromatin at the unset of the S phase (2.0-2.2 C-value), (b) polarization of veiled chromatin (2.2-2.6 C), (c) fibrous chromatin (2.6-3.0 C), chromatin bodies (3.0-3.3 C), early precondensed chromosomes (3.3-3.6). The compaction of Drosophila chromosomes did not reach that of the mammalian cells in the final stage of condensation (3.6-4.0 C). Drosophila chromosomes consist of smaller units called rodlets. Results demonstrate that nucleosomal chromatin ({"}beads on string{"}) does not form a solenoid structure; rather, the topological arrangement consists of meandering and plectonemic loops.",
author = "Gaspar Banfalvi and Gyorgy Trencsenyi and Kinga Ujvarosi and Gabor Nagy and Timea Ombodi and Monika Bedei and Csilla Somogyi and Basnakian, {Alexei G.}",
year = "2007",
month = "1",
doi = "10.1089/dna.2006.0524",
language = "English",
volume = "26",
pages = "55--62",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells

AU - Banfalvi, Gaspar

AU - Trencsenyi, Gyorgy

AU - Ujvarosi, Kinga

AU - Nagy, Gabor

AU - Ombodi, Timea

AU - Bedei, Monika

AU - Somogyi, Csilla

AU - Basnakian, Alexei G.

PY - 2007/1

Y1 - 2007/1

N2 - Earlier, the interphase chromatin structures could not be visualized due to the stickiness of the nuclear material. We have reduced stickiness by the reversal of permeabilization allowing the isolation and microscopic imaging of interphase chromatin structures. By using a high resolution of synchronization, collecting 36 elutriation fractions, we show that major intermediates of chromatin condensation include: (a) decondensed veil-like chromatin at the unset of the S phase (2.0-2.2 C-value), (b) polarization of veiled chromatin (2.2-2.6 C), (c) fibrous chromatin (2.6-3.0 C), chromatin bodies (3.0-3.3 C), early precondensed chromosomes (3.3-3.6). The compaction of Drosophila chromosomes did not reach that of the mammalian cells in the final stage of condensation (3.6-4.0 C). Drosophila chromosomes consist of smaller units called rodlets. Results demonstrate that nucleosomal chromatin ("beads on string") does not form a solenoid structure; rather, the topological arrangement consists of meandering and plectonemic loops.

AB - Earlier, the interphase chromatin structures could not be visualized due to the stickiness of the nuclear material. We have reduced stickiness by the reversal of permeabilization allowing the isolation and microscopic imaging of interphase chromatin structures. By using a high resolution of synchronization, collecting 36 elutriation fractions, we show that major intermediates of chromatin condensation include: (a) decondensed veil-like chromatin at the unset of the S phase (2.0-2.2 C-value), (b) polarization of veiled chromatin (2.2-2.6 C), (c) fibrous chromatin (2.6-3.0 C), chromatin bodies (3.0-3.3 C), early precondensed chromosomes (3.3-3.6). The compaction of Drosophila chromosomes did not reach that of the mammalian cells in the final stage of condensation (3.6-4.0 C). Drosophila chromosomes consist of smaller units called rodlets. Results demonstrate that nucleosomal chromatin ("beads on string") does not form a solenoid structure; rather, the topological arrangement consists of meandering and plectonemic loops.

UR - http://www.scopus.com/inward/record.url?scp=33846885468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846885468&partnerID=8YFLogxK

U2 - 10.1089/dna.2006.0524

DO - 10.1089/dna.2006.0524

M3 - Article

C2 - 17263597

AN - SCOPUS:33846885468

VL - 26

SP - 55

EP - 62

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 1

ER -