In this paper we introduce a new approach towards the modulation of fluorescence resonance energy transfer (FRET). Two stilbazolium dyes (A, D) exhibiting opposite fluorescence responses upon complexation with anionic, water soluble carboxylato-pillararene (WP5) were covalently linked via click chemistry, yielding a FRET-capable ditopic indicator (G). The pseudorotaxane formation of G with WP5 in pure water induced fluorescence enhancement combined with the modulation of the FRET process due to the interaction of the macrocycle with the two fluorophore units.
- Stilbazolium dye
ASJC Scopus subject areas
- Chemical Engineering(all)
- Process Chemistry and Technology