Suppression of yeast RNA polymerase III mutations by the URP2 gene encoding a protein homologous to the mammalian ribosomal protein S20

Sylvie Hermann-Le Denmat, Matthias Sipiczki, Pierre Thuriaux

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Abstract

URP2 was cloned as a multicopy suppressor of several temperature-sensitive mutations defective in RNA polymerase III-dependent transcription, but without effect on mutations affecting RNA polymerase I or II. This single-copy gene encodes a hydrophilic polypeptide of 121 amino acid residues with a predicted molecular mass of 139 kDa and a basic isoelectric point of 97. URP2 is a highly expressed gene, judging from its andbundant messenger RNA and strong codon bias. The Urp2p protein is essential for cell growth, as shown by the lethal phenotype of the urp2::HIS3 null allele. Given its striking similarity to the S20 ribosomal polypeptide of rat (55% identical residues), Urp2p is in all likelihood the yeast form of this polypeptide. Both proteins are significantly related to S10, a component of the small ribosomal subunit of Escherichia coli that is known to operate as a transcriptional elongation factor. The latter observation suggests that the suppressor effect of URP2 may be due to a direct involvement of Urp2p in RNA polymerase III-dependent transcription. Alternatively, the overexpression of Urp2p could bypass a partial preribosomal RNA processing defect associated with RNA polymerase III mutants. URP2 was assigned to the left arm of chromosome VIII, and maps between DUR3 and YLF1. The latter gene product has homology to the E. coli gtp1 gene product, and may define a new family of putative GTP-binding proteins.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of molecular biology
Volume240
Issue number1
DOIs
Publication statusPublished - Jun 30 1994

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Keywords

  • Antitermination
  • GTP-binding
  • Ribosomal RNA
  • S. cerevisiae
  • Transcription

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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