Suppression of 13C satellites in 1H DOSY spectra

Pinelopi Moutzouri, P. Király, Mohammadali Foroozandeh, Andrew R. Phillips, Steven R. Coombes, Mathias Nilsson, Gareth A. Morris

Research output: Contribution to journalArticle

Abstract

Diffusion-ordered spectroscopy (DOSY) is a valuable tool for the analysis of intact mixtures, since it can separate the signals of components according to their apparent diffusion coefficients. However, DOSY experiments are acutely sensitive to spectral quality, and especially to signal overlap, which can lead to misleading apparent diffusion coefficients. Here, we introduce a new NMR experiment to reduce signal overlap in mixtures with a wide range of concentrations, by removing one-bond 13C satellites. In such high dynamic range mixtures, 13C isotopomer signals from major components can overlap with signals from minor components, causing problematic distortions in the diffusion domain of a DOSY spectrum. The new method, Oneshot-iDISPEL, is a combination of the Oneshot and DISPEL experiments, and its performance has been demonstrated on a Greek alcoholic beverage, ouzo, which contains small amounts of anise flavour components and sucrose. Ethanol is a major component, and the suppression of its 13C satellites reduces signal overlap with minor components, offering significant improvement in DOSY spectra.

Original languageEnglish
Pages (from-to)6-11
Number of pages6
JournalJournal of Magnetic Resonance
Volume295
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Spectrum Analysis
retarding
Satellites
Spectroscopy
spectroscopy
diffusion coefficient
beverages
Pimpinella
sucrose
Alcoholic Beverages
Flavors
Experiments
dynamic range
ethyl alcohol
Sucrose
Ethanol
Nuclear magnetic resonance
nuclear magnetic resonance

Keywords

  • C satellites
  • Diffusion
  • DISPEL
  • DOSY
  • NMR
  • Oneshot
  • Ouzo

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Cite this

Moutzouri, P., Király, P., Foroozandeh, M., Phillips, A. R., Coombes, S. R., Nilsson, M., & Morris, G. A. (2018). Suppression of 13C satellites in 1H DOSY spectra. Journal of Magnetic Resonance, 295, 6-11. https://doi.org/10.1016/j.jmr.2018.07.011

Suppression of 13C satellites in 1H DOSY spectra. / Moutzouri, Pinelopi; Király, P.; Foroozandeh, Mohammadali; Phillips, Andrew R.; Coombes, Steven R.; Nilsson, Mathias; Morris, Gareth A.

In: Journal of Magnetic Resonance, Vol. 295, 01.10.2018, p. 6-11.

Research output: Contribution to journalArticle

Moutzouri, P, Király, P, Foroozandeh, M, Phillips, AR, Coombes, SR, Nilsson, M & Morris, GA 2018, 'Suppression of 13C satellites in 1H DOSY spectra', Journal of Magnetic Resonance, vol. 295, pp. 6-11. https://doi.org/10.1016/j.jmr.2018.07.011
Moutzouri P, Király P, Foroozandeh M, Phillips AR, Coombes SR, Nilsson M et al. Suppression of 13C satellites in 1H DOSY spectra. Journal of Magnetic Resonance. 2018 Oct 1;295:6-11. https://doi.org/10.1016/j.jmr.2018.07.011
Moutzouri, Pinelopi ; Király, P. ; Foroozandeh, Mohammadali ; Phillips, Andrew R. ; Coombes, Steven R. ; Nilsson, Mathias ; Morris, Gareth A. / Suppression of 13C satellites in 1H DOSY spectra. In: Journal of Magnetic Resonance. 2018 ; Vol. 295. pp. 6-11.
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