Suppression of meiosis of male germ cells by an antagonist of luteinizing hormone-releasing hormone

B. Szende, Tommie W. Redding, Andrew V. Schally

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Male nude mice were implanted with osmotic minipumps releasing 50 μg of a potent antagonist of luteinizing hormone-releasing hormone (LH-RH) per day {N-Ac-[D-Nal(2)1,D-Phe(pCl)2,D-Pal(3) 3,D-Cit6,D-Ala10]LH-RH} (SB-75) [Nal(2), 3-(2-naphthyl)alanine; Phe(pCl), 4-chlorophenyl-alanine; Pal(3), 3-(3-pyridyl)alanine; Cit, citrulline], or they were treated with s.c. injections of SB-75 (25 μg twice a day). Another group of nude mice received an injection of micro-capsules of the agonist [D-Trp6]LH-RH liberating 25 μg/day. One month after the initiation of treatment, the testicular weights were significantly reduced and the blood testosterone values were at castration levels in all treated groups. Histologically, only the testicles of the mice treated with SB-75 released from minipumps showed a significant decrease of meiosis. The most advanced forms of germ cells were spermatogonia in 26 %, spermatocytes in 17%, and round spermatids in 35% of the seminiferous tubules. Only 22% of the tubules contained elongated spermatids. The suppression of meiotic activity by this modern LH-RH antagonist can possibly be used for the development of methods for male contraception and for the protection of germ cells against the damage caused by cytotoxic drugs and x-radiation.

Original languageEnglish
Pages (from-to)901-903
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number3
Publication statusPublished - 1990

Fingerprint

Meiosis
Germ Cells
Gonadotropin-Releasing Hormone
Spermatids
Nude Mice
Hormone Antagonists
Citrulline
Spermatogonia
Injections
Seminiferous Tubules
Spermatocytes
Castration
Contraception
Alanine
Capsules
Testosterone
Testis
Radiation
Weights and Measures
Pharmaceutical Preparations

Keywords

  • Analogs of luteinizing hormone-releasing hormone
  • Inhibition of spermatogenesis

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Suppression of meiosis of male germ cells by an antagonist of luteinizing hormone-releasing hormone. / Szende, B.; Redding, Tommie W.; Schally, Andrew V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 3, 1990, p. 901-903.

Research output: Contribution to journalArticle

@article{9f19623e17aa40e2a0b6a5fdcfe39bfc,
title = "Suppression of meiosis of male germ cells by an antagonist of luteinizing hormone-releasing hormone",
abstract = "Male nude mice were implanted with osmotic minipumps releasing 50 μg of a potent antagonist of luteinizing hormone-releasing hormone (LH-RH) per day {N-Ac-[D-Nal(2)1,D-Phe(pCl)2,D-Pal(3) 3,D-Cit6,D-Ala10]LH-RH} (SB-75) [Nal(2), 3-(2-naphthyl)alanine; Phe(pCl), 4-chlorophenyl-alanine; Pal(3), 3-(3-pyridyl)alanine; Cit, citrulline], or they were treated with s.c. injections of SB-75 (25 μg twice a day). Another group of nude mice received an injection of micro-capsules of the agonist [D-Trp6]LH-RH liberating 25 μg/day. One month after the initiation of treatment, the testicular weights were significantly reduced and the blood testosterone values were at castration levels in all treated groups. Histologically, only the testicles of the mice treated with SB-75 released from minipumps showed a significant decrease of meiosis. The most advanced forms of germ cells were spermatogonia in 26 {\%}, spermatocytes in 17{\%}, and round spermatids in 35{\%} of the seminiferous tubules. Only 22{\%} of the tubules contained elongated spermatids. The suppression of meiotic activity by this modern LH-RH antagonist can possibly be used for the development of methods for male contraception and for the protection of germ cells against the damage caused by cytotoxic drugs and x-radiation.",
keywords = "Analogs of luteinizing hormone-releasing hormone, Inhibition of spermatogenesis",
author = "B. Szende and Redding, {Tommie W.} and Schally, {Andrew V.}",
year = "1990",
language = "English",
volume = "87",
pages = "901--903",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "3",

}

TY - JOUR

T1 - Suppression of meiosis of male germ cells by an antagonist of luteinizing hormone-releasing hormone

AU - Szende, B.

AU - Redding, Tommie W.

AU - Schally, Andrew V.

PY - 1990

Y1 - 1990

N2 - Male nude mice were implanted with osmotic minipumps releasing 50 μg of a potent antagonist of luteinizing hormone-releasing hormone (LH-RH) per day {N-Ac-[D-Nal(2)1,D-Phe(pCl)2,D-Pal(3) 3,D-Cit6,D-Ala10]LH-RH} (SB-75) [Nal(2), 3-(2-naphthyl)alanine; Phe(pCl), 4-chlorophenyl-alanine; Pal(3), 3-(3-pyridyl)alanine; Cit, citrulline], or they were treated with s.c. injections of SB-75 (25 μg twice a day). Another group of nude mice received an injection of micro-capsules of the agonist [D-Trp6]LH-RH liberating 25 μg/day. One month after the initiation of treatment, the testicular weights were significantly reduced and the blood testosterone values were at castration levels in all treated groups. Histologically, only the testicles of the mice treated with SB-75 released from minipumps showed a significant decrease of meiosis. The most advanced forms of germ cells were spermatogonia in 26 %, spermatocytes in 17%, and round spermatids in 35% of the seminiferous tubules. Only 22% of the tubules contained elongated spermatids. The suppression of meiotic activity by this modern LH-RH antagonist can possibly be used for the development of methods for male contraception and for the protection of germ cells against the damage caused by cytotoxic drugs and x-radiation.

AB - Male nude mice were implanted with osmotic minipumps releasing 50 μg of a potent antagonist of luteinizing hormone-releasing hormone (LH-RH) per day {N-Ac-[D-Nal(2)1,D-Phe(pCl)2,D-Pal(3) 3,D-Cit6,D-Ala10]LH-RH} (SB-75) [Nal(2), 3-(2-naphthyl)alanine; Phe(pCl), 4-chlorophenyl-alanine; Pal(3), 3-(3-pyridyl)alanine; Cit, citrulline], or they were treated with s.c. injections of SB-75 (25 μg twice a day). Another group of nude mice received an injection of micro-capsules of the agonist [D-Trp6]LH-RH liberating 25 μg/day. One month after the initiation of treatment, the testicular weights were significantly reduced and the blood testosterone values were at castration levels in all treated groups. Histologically, only the testicles of the mice treated with SB-75 released from minipumps showed a significant decrease of meiosis. The most advanced forms of germ cells were spermatogonia in 26 %, spermatocytes in 17%, and round spermatids in 35% of the seminiferous tubules. Only 22% of the tubules contained elongated spermatids. The suppression of meiotic activity by this modern LH-RH antagonist can possibly be used for the development of methods for male contraception and for the protection of germ cells against the damage caused by cytotoxic drugs and x-radiation.

KW - Analogs of luteinizing hormone-releasing hormone

KW - Inhibition of spermatogenesis

UR - http://www.scopus.com/inward/record.url?scp=0025020298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025020298&partnerID=8YFLogxK

M3 - Article

C2 - 2405399

AN - SCOPUS:0025020298

VL - 87

SP - 901

EP - 903

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 3

ER -