Suppression of antibody responses to ricin A chain (RTA) by monoclonal anti-RTA antibodies

Vera S. Byers, Eric B. Austin, Jane A. Clegg, Graem Denton, Barbara Gunn, Doreen Hooi, F. Hudecz, Michael R. Price, Robert W. Baldwin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Balb/c mice treated with an immunotoxin constructed by conjugation of murine monoclonal antibody 791T/36 via a disulfide linker to ricin A chain generate a pronounced antibody response to peptide epitopes on ricin A chain. Monoclonal anti-RTA antibodies which recognize peptide epitopes have been developed and these have been used to down-regulate anti-RTA antibody responses in 791T/36-RTA immunotoxin-treated Balb/c mice. Of the five MAB tests, two (608/7 and 596/134) proved most effective, inhibiting anti-RTA antibody formation by up to 73%. MAB treatment was effective when initiated up to 3 days after immunotoxin treatment. Pharmacokinetic studies with 791T/36-RTA have shown that the immunotoxin is rapidly eliminated from the circulation, with no more than 4% remaining in blood after 24 hr. It is proposed that the down-regulation of anti-RTA antibodies is effected by MAB interfering with antigen processing.

Original languageEnglish
Pages (from-to)406-414
Number of pages9
JournalJournal of Clinical Immunology
Volume13
Issue number6
DOIs
Publication statusPublished - Nov 1993

Fingerprint

Ricin
Immunotoxins
Antibody Formation
Anti-Idiotypic Antibodies
Monoclonal Antibodies
Epitopes
Down-Regulation
Peptides
Antigen Presentation
Disulfides
Pharmacokinetics

Keywords

  • antibody responses
  • immunoregulation
  • monoclonal antibodies
  • Ricin A chain

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Byers, V. S., Austin, E. B., Clegg, J. A., Denton, G., Gunn, B., Hooi, D., ... Baldwin, R. W. (1993). Suppression of antibody responses to ricin A chain (RTA) by monoclonal anti-RTA antibodies. Journal of Clinical Immunology, 13(6), 406-414. https://doi.org/10.1007/BF00920016

Suppression of antibody responses to ricin A chain (RTA) by monoclonal anti-RTA antibodies. / Byers, Vera S.; Austin, Eric B.; Clegg, Jane A.; Denton, Graem; Gunn, Barbara; Hooi, Doreen; Hudecz, F.; Price, Michael R.; Baldwin, Robert W.

In: Journal of Clinical Immunology, Vol. 13, No. 6, 11.1993, p. 406-414.

Research output: Contribution to journalArticle

Byers, VS, Austin, EB, Clegg, JA, Denton, G, Gunn, B, Hooi, D, Hudecz, F, Price, MR & Baldwin, RW 1993, 'Suppression of antibody responses to ricin A chain (RTA) by monoclonal anti-RTA antibodies', Journal of Clinical Immunology, vol. 13, no. 6, pp. 406-414. https://doi.org/10.1007/BF00920016
Byers, Vera S. ; Austin, Eric B. ; Clegg, Jane A. ; Denton, Graem ; Gunn, Barbara ; Hooi, Doreen ; Hudecz, F. ; Price, Michael R. ; Baldwin, Robert W. / Suppression of antibody responses to ricin A chain (RTA) by monoclonal anti-RTA antibodies. In: Journal of Clinical Immunology. 1993 ; Vol. 13, No. 6. pp. 406-414.
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