Suppressed neuronal activity and concurrent arteriolar vasoconstriction may explain negative blood oxygenation level-dependent signal

Anna Devor, Peifang Tian, Nozomi Nishimura, Ivan C. Teng, Elizabeth M.C. Hillman, S. N. Narayanan, Istvan Ulbert, David A. Boas, David Kleinfeld, Anders M. Dale

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250 Citations (Scopus)

Abstract

Synaptic transmission initiates a cascade of signal transduction events that couple neuronal activity to local changes in blood flow and oxygenation. Although a number of vasoactive molecules and specific cell types have been implicated, the transformation of stimulus-induced activation of neuronal circuits to hemodynamic changes is still unclear. We use somatosensory stimulation and a suite of in vivo imaging tools to study neurovascular coupling in rat primary somatosensory cortex. Our stimulus evoked a central region of net neuronal depolarization surrounded by net hyperpolarization. Hemodynamic measurements revealed that predominant depolarization corresponded to an increase in oxygenation, whereas predominant hyperpolarization corresponded to a decrease in oxygenation. On the microscopic level of single surface arterioles, the response was composed of a combination of dilatory and constrictive phases. Critically, the relative strength of vasoconstriction covaried with the relative strength of oxygenation decrease and neuronal hyperpolarization. These results suggest that a neuronal inhibition and concurrent arteriolar vasoconstriction correspond to a decrease in blood oxygenation, which would be consistent with a negative blood oxygenation level-dependent functional magnetic resonance imaging signal.

Original languageEnglish
Pages (from-to)4452-4459
Number of pages8
JournalJournal of Neuroscience
Volume27
Issue number16
DOIs
Publication statusPublished - Apr 18 2007

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Keywords

  • Electrode array
  • Hemodynamic
  • Intrinsic imaging
  • Somatosensory
  • Two-photon
  • Voltage-sensitive dyes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Devor, A., Tian, P., Nishimura, N., Teng, I. C., Hillman, E. M. C., Narayanan, S. N., Ulbert, I., Boas, D. A., Kleinfeld, D., & Dale, A. M. (2007). Suppressed neuronal activity and concurrent arteriolar vasoconstriction may explain negative blood oxygenation level-dependent signal. Journal of Neuroscience, 27(16), 4452-4459. https://doi.org/10.1523/JNEUROSCI.0134-07.2007