Superoxide anion radicals generated by methylviologen in photosystem I damage photosystem II

Anja Krieger-Liszkay, P. Kós, É. Hideg

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The effect of superoxide anion radicals on the photosynthetic electron transport chain was studied in leaves and isolated thylakoids from tobacco. Superoxide was generated by methylviologen (MV) in the light at the acceptor side of photosystem I (PSI). In isolated thylakoids, the largest damage was observed at the level of the water-splitting activity in photosystem II (PSII), whereas PSI was hardly affected at the light intensities used. Addition of reactive oxygen scavengers protected PSII against damage. In leaves in the presence of MV, the quantum yield of PSII decreased during illumination whereas the size of the P700 signal remained constant. There was no D1 protein loss in leaves illuminated in the presence of MV and lincomycin, but a modification to a slightly higher molecular mass was observed. These data show that PSII is more sensitive to superoxide or superoxide-derived reactive oxygen species (ROS) than PSI. In our experiments, this susceptibility was not because of any action of the ROS on the translation of the D1 protein or on the repair cycle of photosystem.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalPhysiologia Plantarum
Volume142
Issue number1
DOIs
Publication statusPublished - May 2011

Fingerprint

Photosystem I Protein Complex
Photosystem II Protein Complex
photosystem I
Superoxides
superoxide anion
photosystem II
D1 protein
Thylakoids
thylakoids
reactive oxygen species
Reactive Oxygen Species
leaves
lincomycin
protein depletion
free radical scavengers
electron transport chain
Lincomycin
Light
water activity
lighting

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Physiology

Cite this

Superoxide anion radicals generated by methylviologen in photosystem I damage photosystem II. / Krieger-Liszkay, Anja; Kós, P.; Hideg, É.

In: Physiologia Plantarum, Vol. 142, No. 1, 05.2011, p. 17-25.

Research output: Contribution to journalArticle

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