Superoxide anion production of granulocytes in patients with endometrial cancer at presentation and after treatment

Rudolf Lampé, Márk Ormos, Sándor Szucs, R. Ádány, Edit Szikszay, Robert Póka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: In current medical science the reactive oxygen intermediates play an ever more important role. Methods: The authors analysed superoxide anion production of polymorphonuclear leukocytes (PMNLs) in 30 blood samples from endometrial carcinoma patients. They measured it before the complex treatment and in nine cases at least 1 year after finishing the treatment. The results were compared with healthy controls. Phorbol dibutyrate stimulated superoxide anion production was measured spectrophotometrically as superoxide dismutase inhibitable reduction of ferricytochrome-c absorbance. Results: The mean superoxide anion production of PMNLs of the 31 healthy controls was 1.541 nM/min/105 cells (S.D. = 0.201 nM/min/105 cells). Superoxide anion production of samples from endometrial cancer patients was much lower. The superoxide anion production of granulocytes in the early stage of endometrial cancer was lower (1.112 nM/min/105 cells) as in the controls. There was no essential difference in the superoxide production of patients with different depths of myometrial infiltration. After treatment, the superoxide anion production of the granulocytes of the clinically tumour-free patient had substantially progressed (1.357 nM/min/105 cells), but it was henceforth lower than the control. Conclusions: According to our results, damage to the non-specific immunity is already advanced at the earliest stage of endometrial cancer. Further examinations are needed to decide how to normalise the superoxide production of granulocytes, and whether it has any importance in prevention and therapy.

Original languageEnglish
Pages (from-to)231-234
Number of pages4
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume131
Issue number2
DOIs
Publication statusPublished - Apr 2007

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Endometrial Neoplasms
Granulocytes
Superoxides
Therapeutics
Neutrophils
Cytochromes c
Innate Immunity
Superoxide Dismutase
Oxygen
Neoplasms

Keywords

  • Endometrial cancer
  • Granulocytes
  • Superoxide anion

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Superoxide anion production of granulocytes in patients with endometrial cancer at presentation and after treatment. / Lampé, Rudolf; Ormos, Márk; Szucs, Sándor; Ádány, R.; Szikszay, Edit; Póka, Robert.

In: European Journal of Obstetrics Gynecology and Reproductive Biology, Vol. 131, No. 2, 04.2007, p. 231-234.

Research output: Contribution to journalArticle

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AB - Background: In current medical science the reactive oxygen intermediates play an ever more important role. Methods: The authors analysed superoxide anion production of polymorphonuclear leukocytes (PMNLs) in 30 blood samples from endometrial carcinoma patients. They measured it before the complex treatment and in nine cases at least 1 year after finishing the treatment. The results were compared with healthy controls. Phorbol dibutyrate stimulated superoxide anion production was measured spectrophotometrically as superoxide dismutase inhibitable reduction of ferricytochrome-c absorbance. Results: The mean superoxide anion production of PMNLs of the 31 healthy controls was 1.541 nM/min/105 cells (S.D. = 0.201 nM/min/105 cells). Superoxide anion production of samples from endometrial cancer patients was much lower. The superoxide anion production of granulocytes in the early stage of endometrial cancer was lower (1.112 nM/min/105 cells) as in the controls. There was no essential difference in the superoxide production of patients with different depths of myometrial infiltration. After treatment, the superoxide anion production of the granulocytes of the clinically tumour-free patient had substantially progressed (1.357 nM/min/105 cells), but it was henceforth lower than the control. Conclusions: According to our results, damage to the non-specific immunity is already advanced at the earliest stage of endometrial cancer. Further examinations are needed to decide how to normalise the superoxide production of granulocytes, and whether it has any importance in prevention and therapy.

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