Superovulation using recombinant human FSH and ultrasound-guided transabdominal follicular aspiration in baboon (Papio anubis)

S. Cseh, J. Corselli, P. Chan, L. Bailey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The response of baboon females to a modified human ovarian stimulation protocol incorporating start of pituitary suppression in the luteal phase of the cycle with a GnRH agonist (GnRHa) and recombinant human FSH (rhFSH) was studied. A long-acting GnRHa implant supplying goserelin acetate was administered s.c. to six adult female baboons experiencing regular menstrual cycles (33-34 days) on days 22-24 of the cycle. Follicular development was monitored by transabdominal ultrasonography and serum levels of E2 and progesterone (P4) and rhFSH were determined by ELISA. Menses occurred 9-10 days after GnRHa administration. Daily i.m. administration of 75 IU rhFSH commenced 9-10 days after menses and continued for 9-10 days. When most follicles were ≥5 mm diameter and serum E2 had reached its maximum level, 2000 IU hCG was administered i.m. to induce follicle maturation.Transabdominal ultrasound-guided follicular aspiration of follicles ≥2 mm diameter was performed 30-34 h after hCG administration. One baboon did not show an adequate response to rhFSH stimulation. This animal did not receive further treatment and no data for it are presented. The number of follicles aspirated was 21 ± 4 and 17.2 ± 3.8 oocytes were recovered per animal with an average recovery rate of 82% (86/105). The number of oocytes collected from five animals were 14, 21, 16, 15, and 20 (n = 86). Most of the oocytes recovered were in metaphase II and 3 h after recovery 91% (78/86) were considered suitable for in vitro fertilization. It was concluded that recombinant human FSH can successfully induce follicular recruitment and oocyte maturation in baboon females during pituitary suppression with a GnRHa.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalAnimal Reproduction Science
Volume70
Issue number3-4
DOIs
Publication statusPublished - Apr 15 2002

Fingerprint

Papio anubis
Human Follicle Stimulating Hormone
Superovulation
superovulation
Papio
Gonadotropin-Releasing Hormone
Oocytes
agonists
oocytes
Menstruation
menstruation
human chorionic gonadotropin
Goserelin
Ovulation Induction
Luteal Phase
Fertilization in Vitro
Metaphase
Menstrual Cycle
animals
menstrual cycle

Keywords

  • Assisted reproduction
  • Baboon
  • Conservation
  • Follicular aspiration
  • GnRH agonist
  • Recombinant human FSH
  • Superovulation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Genetics

Cite this

Superovulation using recombinant human FSH and ultrasound-guided transabdominal follicular aspiration in baboon (Papio anubis). / Cseh, S.; Corselli, J.; Chan, P.; Bailey, L.

In: Animal Reproduction Science, Vol. 70, No. 3-4, 15.04.2002, p. 287-293.

Research output: Contribution to journalArticle

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