Supercritical fluid extraction of Vitex agnus castus fruit

D. Cossuta, B. Simándi, E. Vági, J. Hohmann, A. Prechl, É Lemberkovics, Á Kéry, T. Keve

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The effects of supercritical fluid extraction (SFE) conditions (pressure and temperature) on extraction yield and recoveries of biologically active components were studied using a 32 full factorial design. Pilot plant SFE experiments were performed in a 5 × 10-3 m3 volume high-pressure vessel. The pressure and temperature were varied over the ranges of 100-450 bar and 40-60 °C, respectively. The yield and recoveries were compared to those obtained with n-hexane and ethanol (96%, v/v) extractions. The extract samples were analysed by TLC, TLC-densitometry, GC and HPLC methods. The obtained yields changed between 1.9 and 65.7 g kg-1, according to the solvent power of the supercritical fluid. The recoveries of the different minor components were (g minor components kg-1 dried raw material): 0.06-1.06 rotundifurane, 0.02-1.08 β-sitosterol, 0.04-0.63 β-amyrin, 0.87-2.71 casticin. By evaluation the designed experiments 450 bar and 45 °C were chosen as the best conditions within the ranges investigated.

Original languageEnglish
Pages (from-to)188-194
Number of pages7
JournalJournal of Supercritical Fluids
Volume47
Issue number2
DOIs
Publication statusPublished - Dec 1 2008

Keywords

  • Casticin
  • Diterpene
  • Supercritical fluid extraction
  • Triterpene
  • Vitex agnus castus

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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