Supercritical fluid extraction and convergence chromatographic determination of parthenolide in Tanacetum parthenium L.

Experimental design, modeling and optimization

Krisztina Végh, Ágnes Alberti, Eszter Riethmüller, Anita Tóth, S. Béni, A. Kéry

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Feverfew (Tanacetum parthenium L., Asteraceae) is a perennial medicinal plant which has been used to alleviate the symptoms of migraines, headaches and rheumatoid arthritis. The herb contains various potentially active constituents such as sesquiterpene-γ-lactones, flavonoids and volatile oil. The main sesquiterpene-lactone in feverfew is parthenolide which is considered to be responsible for the therapeutical effects. Supercritical CO2 extraction was carried out at different pressures (10-30 MPa), temperatures (40-80 °C) and co-solvent contents (0-10% ethanol) in order to study the extraction yield and the parthenolide recovery of the extracts. Leaves collected before and during flowering and flower heads were investigated. A factorial experiment using a full 33 design was followed during the experiments and response surface methodology was implemented to analyze the influence of the variables and optimize the extraction. The critical values of parthenolide content were found to be 7% EtOH, 22 MPa and 64 °C in case of all three samples. It was determined, that the optimal conditions of the extraction, where the maximum parthenolide content and extract yield can be reached, do not coincide. The highest yield of parthenolide was obtained in the flower heads (0.604 wt.%).

Original languageEnglish
Pages (from-to)84-91
Number of pages8
JournalJournal of Supercritical Fluids
Volume95
DOIs
Publication statusPublished - 2014

Fingerprint

Supercritical fluid extraction
supercritical fluids
Design of experiments
optimization
Sesquiterpenes
Lactones
headache
Flavonoids
arthritis
leaves
Volatile Oils
Ethanol
ethyl alcohol
oils
Experiments
recovery
methodology
Recovery
parthenolide
Temperature

Keywords

  • Convergence chromatography
  • Experimental design
  • Response surface methodology
  • Supercritical fluid extraction
  • Tanacetum parthenium L.

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Chemical Engineering(all)
  • Condensed Matter Physics

Cite this

Supercritical fluid extraction and convergence chromatographic determination of parthenolide in Tanacetum parthenium L. Experimental design, modeling and optimization. / Végh, Krisztina; Alberti, Ágnes; Riethmüller, Eszter; Tóth, Anita; Béni, S.; Kéry, A.

In: Journal of Supercritical Fluids, Vol. 95, 2014, p. 84-91.

Research output: Contribution to journalArticle

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