Super-resolution microscope using a two-color phase plate for generating quasi-Laguerre-Gaussian beam

Yoshinori Iketaki, Nandor Bokor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To achieve high lateral resolution overcoming the diffraction limit, a two-color phase plate (TPP) for generating a quasi-Laguerre-Gaussian beam was applied to super-resolution microscopy (SRM) based on fluorescence depletion. Putting the TPP into a robust optical path in a commercial laser-scanning microscope, we obtained a point spread function with a full width at half maximum three times smaller than diffraction limit. The measured contrast transfer function shows that line and space patterns finer than the diffraction limit were clearly resolved and the image contrast was improved. Since the TPP can easily improve lateral resolution in SRM without any precise adjustment, our setup provides a practical super-resolution microscope.

Original languageEnglish
Pages (from-to)3798-3804
Number of pages7
JournalOptics Communications
Volume285
Issue number18
DOIs
Publication statusPublished - Aug 15 2012

Keywords

  • Fluorescence depletion
  • Laguerre-Gaussian beam
  • Laser-scanning microscope
  • Phase plate
  • Super-resolution microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry
  • Electrical and Electronic Engineering

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