99mTc-labelled nanosystem as tumour imaging agent for SPECT and SPECT/CT modalities

András Polyák, István Hajdu, Magdolna Bodnár, György Trencsényi, Zita Pöstényi, Veronika Haász, Gergely Jánoki, Gyözö A. Jánoki, Lajos Balogh, János Borbély

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We report the synthesis, in vitro and in vivo investigation of folate-targeted, biocompatible, biodegradable self-assembled nanoparticles radiolabelled with 99mTc, as potential new SPECT or SPECT/CT imaging agent. Nanoparticles with hydrodynamic size in the range of 75-200 nm were prepared by self-assembly of chitosan and folated poly-γ-glutamic acid, and then radiolabelled with 99mTc. The nanoparticles target tumour cells overexpressing folate receptors and internalize specifically into them to realize early tumour diagnosis detected by SPECT and SPECT/CT modalities. Rat hepatocellular carcinoma cells were used as model system. Cell specificity and tumour targeting efficacy of these nanosystems were investigated in vitro, and in vivo using SPECT and fusion nanoSPECT/CT imaging. In vitro results showed that the radiolabeled nanosystem was efficiently internalized by tumour cells. Whole-body biodistribution of the new radiolabelled, folate-targeted nanoparticles revealed higher uptake in the tumorous kidney compared to the non-tumorous contralateral side. Uptake by the lungs and thyroids was negligible, which confirmed the stability of the nanoparticles in vivo. In vivo SPECT and SPECT/CT imaging visually reinforced the uptake results and were in accordance with the biodistribution data: the new nanoparticles as a targeted contrast agent improve tumour targeting and are able to detect folate-receptor-overexpressing tumours in animal models with enhanced contrast.

Original languageEnglish
Pages (from-to)10-17
Number of pages8
JournalInternational Journal of Pharmaceutics
Volume449
Issue number1-2
DOIs
Publication statusPublished - Jun 5 2013

Keywords

  • Folate-targeted
  • In vivo
  • Nanoparticles
  • Radiolabeled
  • SPECT
  • SPECT/CT
  • Tc-99m

ASJC Scopus subject areas

  • Pharmaceutical Science

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  • Cite this

    Polyák, A., Hajdu, I., Bodnár, M., Trencsényi, G., Pöstényi, Z., Haász, V., Jánoki, G., Jánoki, G. A., Balogh, L., & Borbély, J. (2013). 99mTc-labelled nanosystem as tumour imaging agent for SPECT and SPECT/CT modalities. International Journal of Pharmaceutics, 449(1-2), 10-17. https://doi.org/10.1016/j.ijpharm.2013.03.049