99mTc-Labeled RGD-Polyethylenimine Conjugates with Entrapped Gold Nanoparticles in the Cavities for Dual-Mode SPECT/CT Imaging of Hepatic Carcinoma

Benqing Zhou, Ruizhi Wang, Feng Chen, Lingzhou Zhao, Peng Wang, Xin Li, I. Bányai, Qiang Ouyang, Xiangyang Shi, Mingwu Shen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We report the construction and characterization of 99mTc-labeled arginine-glycine-aspartic acid (RGD)-polyethylenimine (PEI) conjugates with entrapped gold nanoparticles in the cavities (RGD-99mTc-Au PENPs) for dual-mode single-photon emission computed tomography (SPECT)/computed tomography (CT) imaging of an orthotopic hepatic carcinoma model. In this study, PEI was successively decorated with diethylenetriaminepentaacetic acid, poly(ethylene glycol) (PEG), and PEGylated RGD segments, and was utilized as an effective nanoplatform to entrap Au NPs and to be labeled with 99mTc. We showed that the designed RGD-99mTc-Au PENPs displayed desirable colloidal stability and radiostability, and cytocompatibility in the investigated concentration range, and could be specifically uptaken by αvβ3 integrin-overexpressing liver cancer cells in vitro. In vivo CT and SPECT imaging results indicated that the particles were able to be accumulated within an orthotopic hepatic carcinoma and displayed both CT and SPECT contrast enhancement in the tumor tissue. With the proven biocompatibility in vivo via histological examinations, the designed RGD-99mTc-Au PENPs may be potentially employed as an effective nanoprobe for a highly efficient dual-mode SPECT/CT imaging of various αvβ3 integrin-overexpressing tumors.

Original languageEnglish
Pages (from-to)6146-6154
Number of pages9
JournalACS Applied Materials and Interfaces
Volume10
Issue number7
DOIs
Publication statusPublished - Feb 21 2018

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Single photon emission computed tomography
Polyethyleneimine
Gold
Tomography
Nanoparticles
Imaging techniques
Integrins
Polyethylene glycols
Tumors
Nanoprobes
Arginine
Acids
Biocompatibility
Aspartic Acid
Liver
Glycine
Amino acids
Cells
Tissue

Keywords

  • Tc
  • gold nanoparticles
  • hepatic carcinoma
  • polyethylenimine
  • SPECT/CT imaging

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

99mTc-Labeled RGD-Polyethylenimine Conjugates with Entrapped Gold Nanoparticles in the Cavities for Dual-Mode SPECT/CT Imaging of Hepatic Carcinoma. / Zhou, Benqing; Wang, Ruizhi; Chen, Feng; Zhao, Lingzhou; Wang, Peng; Li, Xin; Bányai, I.; Ouyang, Qiang; Shi, Xiangyang; Shen, Mingwu.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 7, 21.02.2018, p. 6146-6154.

Research output: Contribution to journalArticle

Zhou, Benqing ; Wang, Ruizhi ; Chen, Feng ; Zhao, Lingzhou ; Wang, Peng ; Li, Xin ; Bányai, I. ; Ouyang, Qiang ; Shi, Xiangyang ; Shen, Mingwu. / 99mTc-Labeled RGD-Polyethylenimine Conjugates with Entrapped Gold Nanoparticles in the Cavities for Dual-Mode SPECT/CT Imaging of Hepatic Carcinoma. In: ACS Applied Materials and Interfaces. 2018 ; Vol. 10, No. 7. pp. 6146-6154.
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