[3H]Naloxone binding in the brain of the domestic chick (Gallus domesticus) determined by in vitro quantitative autoradiography

Andras Csillag, Rachel C. Bourne, Mihaly Kalman, Margaret I. Boxer, Michael G. Stewart

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The distribution of [3H]naloxone binding sites was investigated in the brains of one-day-old domestic chicks using quantitative receptor autoradiography. Among the forebrain regions, the greatest levels of opiate binding were found in hyperstriatum dorsale, hyperstriatum ventrale, hyperstriatum intercalatum supremum and neostriatum. Intermediate levels were found throughout the paleostriatal regions, septum, thalamus, archistriatum, hyperstriatum accessorium and area parahippocampalis whilst in hippocampus and ectostriatum the density of [3H]naloxone binding sites was low. In the hindbrain, high levels of opiate binding were found in optic tectum whereas in cerebellum the density of binding sites was barely above background. The greatest densities of opiate binding appear to coincide with regions found to be involved in sensory processing and memory storage.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalBrain research
Volume479
Issue number2
DOIs
Publication statusPublished - Feb 13 1989

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Keywords

  • Avian brain
  • Domestic chick
  • Naloxone binding
  • Opiates
  • Quantitative autoradiography
  • [H]-

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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