[3H]Histamine uptake and release by astrocytes from rat brain: Effects of sodium deprivation, high potassium, and potassium channel blockers

Zsuzsanna Huszti, Péter Imrik, Emilia Madarász

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Histamine transport has been characterized in cultured astroglial cells of rat brain. The kinetics of [3H]-histamine uptake yielded a Km of 0.19±0.03 μM and a Vmax of 3.12±0.75 pmol×mg protein-1×min-1. Transport system revealed high affinity for histamine and an approximately ten times higher capacity than that shown in cultured glial cells of chick embryonic brain. Ouabain which interferes with utilization of ATP to generate ion gradients, and the replacement of Na+ with choline inhibited the initial rate of uptake showing a strong Na+-dependency and suggesting the presence of a tightly coupled sodium/histamine symporter. Dissipation of K+-gradient (in>out) by high K+ or by K+-channel blockers, BaCl2, (100 μM), quinine (100 μM) or Sparteine (20 μM) produced also remarkable inhibitions in the uptake of [3H]-histamine. Impromidine, a structural histamine-analogue could inhibit the uptake non-competitively in a range of concentrations of 1 to 10 μM with a Ki value of 2.8 μM, indicating the specificity of the uptake. [3H]histamine uptake measurements carried out by using a suspension of dissociated hypothalamic cells, of rat brain showed a strong gliotoxin-sensitivity and yielded a Km of 0.33±0.08 μM; and a Vmax of 2.65±0.35 pmoles×mg protein-1×min-1. The uptake could be reversed by incubating the cells in histamine-free Krebs medium. The [3H]histamine efflux was sensitive to Na+ omission, ouabain treatment and high K+ or K+ channel blockers, resulting in marked elevations in the efflux. Data indicate that glial uptake of histamine is a high affinity, Na+-dependent and electrogenic, driven by an inward-oriented sodium ion gradient and an outward-oriented potassium ion gradient and functions as part of histamine inactivation, at least in a shunt mechanism.

Original languageEnglish
Pages (from-to)1249-1256
Number of pages8
JournalNeurochemical research
Volume19
Issue number10
DOIs
Publication statusPublished - Oct 1 1994

Keywords

  • Histamine transport
  • cultured astroglial cells
  • rat brain

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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