[3H]dopamine release in striatum in response to cortical stimulation in a corticostriatal slice preparation

Zsolt Juranyi, Michael J. Zigmond, L. Hársing

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A new method has been developed to investigate corticostriatal glutamatergic influence on [3H]dopamine release in striatum in complex corticostriatal slice preparation in vitro. Horizontal slices containing the striatum and the adjacent prefrontal cortex of rat brain were cut in a plane that maintains corticostriatal connections. After incubation with [3H]dopamine, slices were submerged in a two-compartment bath so that the cortical region was contained entirely in one compartment, corpus callosum passed through a silicone greased slot, and the striatal region was contained in the other compartment. A cannula was placed just above the striatal part of the slice and effluent was collected with a peristaltic pump, released tritiated materials were counted with a liquid scintillation counter. Electric field stimulation of cortex increased the release of [3H]dopamine in the striatum. Bicuculline (1 mM) increased the basal and stimulated release of [3H]dopamine in the striatum in response to cortical stimulation of cortex indicating the GABAergic control on dopamine release. This method allows investigation of the effect of cortical stimulation on glutamate-dopamine-GABA interactions in the striatum in vitro that might help to understand better the neurochemical background of schizophrenia or Parkinson's disease.

Original languageEnglish
Pages (from-to)57-67
Number of pages11
JournalJournal of Neuroscience Methods
Volume126
Issue number1
DOIs
Publication statusPublished - Jun 15 2003

Fingerprint

Dopamine
Corpus Striatum
Scintillation Counting
Bicuculline
Corpus Callosum
Silicones
Prefrontal Cortex
Baths
gamma-Aminobutyric Acid
Electric Stimulation
Parkinson Disease
Glutamic Acid
Schizophrenia
Brain
In Vitro Techniques

Keywords

  • Dopamine
  • Neurotransmitter
  • Release
  • Striatum
  • Wedge

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

[3H]dopamine release in striatum in response to cortical stimulation in a corticostriatal slice preparation. / Juranyi, Zsolt; Zigmond, Michael J.; Hársing, L.

In: Journal of Neuroscience Methods, Vol. 126, No. 1, 15.06.2003, p. 57-67.

Research output: Contribution to journalArticle

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