1. The binding of [3H]-imipramine to brain membrane preparations of the insects, Periplaneta americana, Locusta migratoria and the mollusc, Helix pomatia was investigated. 2. [3H]-Imipramine binding is similar in invertebrates and vertebrates although, in insects, the binding is independent of sodium. 3. In the three invertebrate systems studied, [3H]-imipramine binding was inhibited by 5-hydroxytryptamine (5-HT) only at high concentrations (100 μM 5-HT caused 20-30% inhibition in insects and 10% inhibition in snail). 4. Studies on binding and incorporation of [3H]-imipramine and [3H]-5-hydroxytryptamine indicate that the imipramine binding site differs from the 5-hydroxytryptamine recognition site of uptake. 5. The [3H]-imipramine binding sites are not specific for 5-hydroxytryptamine and have similar affinity for dopamine.
|Number of pages||4|
|Journal||Comparative Biochemistry and Physiology. Part C, Comparative|
|Publication status||Published - Sep 1992|
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