3H-imipramine binding in membrane preparations from brains of insects (Periplaneta americana, Locusta migratoria) and mollusc (Helix pomatia)

Roger G.H. Downer, László Hiripi

Research output: Contribution to journalArticle

Abstract

1. The binding of [3H]-imipramine to brain membrane preparations of the insects, Periplaneta americana, Locusta migratoria and the mollusc, Helix pomatia was investigated. 2. [3H]-Imipramine binding is similar in invertebrates and vertebrates although, in insects, the binding is independent of sodium. 3. In the three invertebrate systems studied, [3H]-imipramine binding was inhibited by 5-hydroxytryptamine (5-HT) only at high concentrations (100 μM 5-HT caused 20-30% inhibition in insects and 10% inhibition in snail). 4. Studies on binding and incorporation of [3H]-imipramine and [3H]-5-hydroxytryptamine indicate that the imipramine binding site differs from the 5-hydroxytryptamine recognition site of uptake. 5. The [3H]-imipramine binding sites are not specific for 5-hydroxytryptamine and have similar affinity for dopamine.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume103
Issue number1
DOIs
Publication statusPublished - Sep 1992

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ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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