3H GABA uptake and acetylcholinesterase activity in dissociated cell cultures of the medial hypothalamus

G. Makara, A. Gyevai, Gy Rappay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Medial hypothalamic tissue of 1 to 4 days old rats was dissociated and cultured in vitro for 8-10 days. Neuronal perikarya were demonstrated by supravital methylene blue staining and electron microscopy. Synapses with typical vesicles and subsynaptic thickening were also observed. 3H-GABA was taken up into a small percentage of the cells in the cultures. Neuron like perikarya and long processes accumulated the label while many neurons contained much less activity. Some astroglial and oligodendroglial cells and processes also accumulated GABA. A few neurons in these cultures contained acetylcholinesterase. It is concluded that neurons concentrating GABA and containing acetylcholinesterase are present in the hypothalamus of rats of 1 to 4 days of age and can be maintained in dissociated cell culture.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalActa Biologica Academiae Scientiarum Hungaricae
Volume27
Issue number2-3
Publication statusPublished - 1976

Fingerprint

Middle Hypothalamus
Acetylcholinesterase
acetylcholinesterase
hypothalamus
Cell culture
gamma-Aminobutyric Acid
Neurons
cell culture
Cell Culture Techniques
neurons
vesicle
electron microscopy
Rats
GABAergic Neurons
Methylene Blue
Synapses
Hypothalamus
methylene blue
rats
Electron Microscopy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

3H GABA uptake and acetylcholinesterase activity in dissociated cell cultures of the medial hypothalamus. / Makara, G.; Gyevai, A.; Rappay, Gy.

In: Acta Biologica Academiae Scientiarum Hungaricae, Vol. 27, No. 2-3, 1976, p. 191-197.

Research output: Contribution to journalArticle

@article{94d75161e72643498171b17d3a60d149,
title = "3H GABA uptake and acetylcholinesterase activity in dissociated cell cultures of the medial hypothalamus",
abstract = "Medial hypothalamic tissue of 1 to 4 days old rats was dissociated and cultured in vitro for 8-10 days. Neuronal perikarya were demonstrated by supravital methylene blue staining and electron microscopy. Synapses with typical vesicles and subsynaptic thickening were also observed. 3H-GABA was taken up into a small percentage of the cells in the cultures. Neuron like perikarya and long processes accumulated the label while many neurons contained much less activity. Some astroglial and oligodendroglial cells and processes also accumulated GABA. A few neurons in these cultures contained acetylcholinesterase. It is concluded that neurons concentrating GABA and containing acetylcholinesterase are present in the hypothalamus of rats of 1 to 4 days of age and can be maintained in dissociated cell culture.",
author = "G. Makara and A. Gyevai and Gy Rappay",
year = "1976",
language = "English",
volume = "27",
pages = "191--197",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "2-3",

}

TY - JOUR

T1 - 3H GABA uptake and acetylcholinesterase activity in dissociated cell cultures of the medial hypothalamus

AU - Makara, G.

AU - Gyevai, A.

AU - Rappay, Gy

PY - 1976

Y1 - 1976

N2 - Medial hypothalamic tissue of 1 to 4 days old rats was dissociated and cultured in vitro for 8-10 days. Neuronal perikarya were demonstrated by supravital methylene blue staining and electron microscopy. Synapses with typical vesicles and subsynaptic thickening were also observed. 3H-GABA was taken up into a small percentage of the cells in the cultures. Neuron like perikarya and long processes accumulated the label while many neurons contained much less activity. Some astroglial and oligodendroglial cells and processes also accumulated GABA. A few neurons in these cultures contained acetylcholinesterase. It is concluded that neurons concentrating GABA and containing acetylcholinesterase are present in the hypothalamus of rats of 1 to 4 days of age and can be maintained in dissociated cell culture.

AB - Medial hypothalamic tissue of 1 to 4 days old rats was dissociated and cultured in vitro for 8-10 days. Neuronal perikarya were demonstrated by supravital methylene blue staining and electron microscopy. Synapses with typical vesicles and subsynaptic thickening were also observed. 3H-GABA was taken up into a small percentage of the cells in the cultures. Neuron like perikarya and long processes accumulated the label while many neurons contained much less activity. Some astroglial and oligodendroglial cells and processes also accumulated GABA. A few neurons in these cultures contained acetylcholinesterase. It is concluded that neurons concentrating GABA and containing acetylcholinesterase are present in the hypothalamus of rats of 1 to 4 days of age and can be maintained in dissociated cell culture.

UR - http://www.scopus.com/inward/record.url?scp=0017094815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017094815&partnerID=8YFLogxK

M3 - Article

C2 - 1036000

AN - SCOPUS:0017094815

VL - 27

SP - 191

EP - 197

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 2-3

ER -