[14C]adenine uptake into Bacillus anthracis adenylsuccinate lyase deficient mutant

J. Molnar, B. Pragai

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Abstract

The B. anthracis adenylsuccinate lyase deficient mutant utilized part of labelled adenine for growth and converted part of it to hypoxanthine, Multiplication of the mutant in the presence of 14C adenine ceased at a low level, although a considerable amount of free adenine was still demonstrable in the medium. The sonicated cell suspension of the mutant caused the conversion of labelled adenine to inosine and, subsequently, to hypoxanthine: traces of adenine nucleotides were also formed. The cell extract synthesized adenine nucleotides from adenine in the presence of exogenous ATP. Ribose 5 phosphate enhanced the nucleotide synthesis stimulating effect of ATP. The results suggest that adenosine deaminase activity on the one hand and the exhaustion of the 5 phosphoribosyl pyrophosphate pool on the other may be the factors limiting the mutant's growth, viz. adenine incorporation.

Original languageEnglish
Pages (from-to)219-227
Number of pages9
JournalActa Microbiologica Academiae Scientiarum Hungaricae
Volume21
Issue number3-4
Publication statusPublished - 1974

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Bacillus anthracis
Lyases
Adenine
Hypoxanthine
Adenine Nucleotides
Phosphoribosyl Pyrophosphate
Adenosine Triphosphate
Inosine
Adenosine Deaminase
Growth
Cell Extracts
Suspensions
Nucleotides

ASJC Scopus subject areas

  • Medicine(all)

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[14C]adenine uptake into Bacillus anthracis adenylsuccinate lyase deficient mutant. / Molnar, J.; Pragai, B.

In: Acta Microbiologica Academiae Scientiarum Hungaricae, Vol. 21, No. 3-4, 1974, p. 219-227.

Research output: Contribution to journalArticle

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