Summative interaction between astaxanthin, Ginkgo biloba extract (EGb761) and vitamin C in Suppression of respiratory inflammation

A comparison with ibuprofen

David D. Haines, Balazs Varga, I. Bak, B. Juhász, Fadia F. Mahmoud, Heybatullah Kalantari, R. Gesztelyi, I. Lekli, Attila Czompa, A. Tósaki

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this study, combinations of Ginkgo biloba leaf extract (EGb761) plus the carotenoid antioxidant astaxanthin (ASX) and vitamin C were evaluated for a summative dose effect in the inhibition of asthma-associated inflammation in asthmatic guinea-pigs. Ovalbumin-sensitized Hartley guinea-pigs challenged with ovalbumin aerosol to induce asthma, were administered EGb761, ASX, vitamin C or ibuprofen. Following killing, bronchoalveolar lavage (BAL) fluid was evaluated for inflammatory cell infiltrates and lung tissue cyclic nucleotide content. Each parameter measured was significantly altered to a greater degree by drug combinations, than by each component acting independently. An optimal combination was identified that included astaxanthin (10 mg/kg), vitamin C (200 mg/kg) and EGb761 (10 mg/kg), resulting in counts of eosinophils and neutrophils each 1.6-fold lower; macrophages 1.8-fold lower, cAMP 1.4-fold higher; and cGMP 2.04-fold higher than levels in untreated, asthmatic animals (p <0.05). In conclusion, EGb761, ASX and vitamin C are shown here to interact summatively to suppress inflammation with efficacy equal to or better than ibuprofen, a widely used non-steroidal antiinflammatory drug (NSAID). Such combinations of non-toxic phytochemicals constitute powerful tools for the prevention of onset of acute and chronic inflammatory disease if consumed regularly by healthy individuals; and may also augment the effectiveness of therapy for those with established illness.

Original languageEnglish
Pages (from-to)128-136
Number of pages9
JournalPhytotherapy Research
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Ginkgo biloba
Ibuprofen
Ascorbic Acid
Inflammation
Ovalbumin
Guinea Pigs
Asthma
Cyclic Nucleotides
Bronchoalveolar Lavage Fluid
Phytochemicals
Drug Combinations
Carotenoids
Aerosols
Eosinophils
Neutrophils
Chronic Disease
Anti-Inflammatory Agents
Antioxidants
Macrophages
Lung

Keywords

  • astaxanthin
  • cAMP
  • cGMP
  • ginkgolide
  • ibuprofen
  • inflammation

ASJC Scopus subject areas

  • Pharmacology

Cite this

Summative interaction between astaxanthin, Ginkgo biloba extract (EGb761) and vitamin C in Suppression of respiratory inflammation : A comparison with ibuprofen. / Haines, David D.; Varga, Balazs; Bak, I.; Juhász, B.; Mahmoud, Fadia F.; Kalantari, Heybatullah; Gesztelyi, R.; Lekli, I.; Czompa, Attila; Tósaki, A.

In: Phytotherapy Research, Vol. 25, No. 1, 01.2011, p. 128-136.

Research output: Contribution to journalArticle

@article{70dfe313338b4749a3fa4d57ac3a31c8,
title = "Summative interaction between astaxanthin, Ginkgo biloba extract (EGb761) and vitamin C in Suppression of respiratory inflammation: A comparison with ibuprofen",
abstract = "In this study, combinations of Ginkgo biloba leaf extract (EGb761) plus the carotenoid antioxidant astaxanthin (ASX) and vitamin C were evaluated for a summative dose effect in the inhibition of asthma-associated inflammation in asthmatic guinea-pigs. Ovalbumin-sensitized Hartley guinea-pigs challenged with ovalbumin aerosol to induce asthma, were administered EGb761, ASX, vitamin C or ibuprofen. Following killing, bronchoalveolar lavage (BAL) fluid was evaluated for inflammatory cell infiltrates and lung tissue cyclic nucleotide content. Each parameter measured was significantly altered to a greater degree by drug combinations, than by each component acting independently. An optimal combination was identified that included astaxanthin (10 mg/kg), vitamin C (200 mg/kg) and EGb761 (10 mg/kg), resulting in counts of eosinophils and neutrophils each 1.6-fold lower; macrophages 1.8-fold lower, cAMP 1.4-fold higher; and cGMP 2.04-fold higher than levels in untreated, asthmatic animals (p <0.05). In conclusion, EGb761, ASX and vitamin C are shown here to interact summatively to suppress inflammation with efficacy equal to or better than ibuprofen, a widely used non-steroidal antiinflammatory drug (NSAID). Such combinations of non-toxic phytochemicals constitute powerful tools for the prevention of onset of acute and chronic inflammatory disease if consumed regularly by healthy individuals; and may also augment the effectiveness of therapy for those with established illness.",
keywords = "astaxanthin, cAMP, cGMP, ginkgolide, ibuprofen, inflammation",
author = "Haines, {David D.} and Balazs Varga and I. Bak and B. Juh{\'a}sz and Mahmoud, {Fadia F.} and Heybatullah Kalantari and R. Gesztelyi and I. Lekli and Attila Czompa and A. T{\'o}saki",
year = "2011",
month = "1",
doi = "10.1002/ptr.3160",
language = "English",
volume = "25",
pages = "128--136",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Summative interaction between astaxanthin, Ginkgo biloba extract (EGb761) and vitamin C in Suppression of respiratory inflammation

T2 - A comparison with ibuprofen

AU - Haines, David D.

AU - Varga, Balazs

AU - Bak, I.

AU - Juhász, B.

AU - Mahmoud, Fadia F.

AU - Kalantari, Heybatullah

AU - Gesztelyi, R.

AU - Lekli, I.

AU - Czompa, Attila

AU - Tósaki, A.

PY - 2011/1

Y1 - 2011/1

N2 - In this study, combinations of Ginkgo biloba leaf extract (EGb761) plus the carotenoid antioxidant astaxanthin (ASX) and vitamin C were evaluated for a summative dose effect in the inhibition of asthma-associated inflammation in asthmatic guinea-pigs. Ovalbumin-sensitized Hartley guinea-pigs challenged with ovalbumin aerosol to induce asthma, were administered EGb761, ASX, vitamin C or ibuprofen. Following killing, bronchoalveolar lavage (BAL) fluid was evaluated for inflammatory cell infiltrates and lung tissue cyclic nucleotide content. Each parameter measured was significantly altered to a greater degree by drug combinations, than by each component acting independently. An optimal combination was identified that included astaxanthin (10 mg/kg), vitamin C (200 mg/kg) and EGb761 (10 mg/kg), resulting in counts of eosinophils and neutrophils each 1.6-fold lower; macrophages 1.8-fold lower, cAMP 1.4-fold higher; and cGMP 2.04-fold higher than levels in untreated, asthmatic animals (p <0.05). In conclusion, EGb761, ASX and vitamin C are shown here to interact summatively to suppress inflammation with efficacy equal to or better than ibuprofen, a widely used non-steroidal antiinflammatory drug (NSAID). Such combinations of non-toxic phytochemicals constitute powerful tools for the prevention of onset of acute and chronic inflammatory disease if consumed regularly by healthy individuals; and may also augment the effectiveness of therapy for those with established illness.

AB - In this study, combinations of Ginkgo biloba leaf extract (EGb761) plus the carotenoid antioxidant astaxanthin (ASX) and vitamin C were evaluated for a summative dose effect in the inhibition of asthma-associated inflammation in asthmatic guinea-pigs. Ovalbumin-sensitized Hartley guinea-pigs challenged with ovalbumin aerosol to induce asthma, were administered EGb761, ASX, vitamin C or ibuprofen. Following killing, bronchoalveolar lavage (BAL) fluid was evaluated for inflammatory cell infiltrates and lung tissue cyclic nucleotide content. Each parameter measured was significantly altered to a greater degree by drug combinations, than by each component acting independently. An optimal combination was identified that included astaxanthin (10 mg/kg), vitamin C (200 mg/kg) and EGb761 (10 mg/kg), resulting in counts of eosinophils and neutrophils each 1.6-fold lower; macrophages 1.8-fold lower, cAMP 1.4-fold higher; and cGMP 2.04-fold higher than levels in untreated, asthmatic animals (p <0.05). In conclusion, EGb761, ASX and vitamin C are shown here to interact summatively to suppress inflammation with efficacy equal to or better than ibuprofen, a widely used non-steroidal antiinflammatory drug (NSAID). Such combinations of non-toxic phytochemicals constitute powerful tools for the prevention of onset of acute and chronic inflammatory disease if consumed regularly by healthy individuals; and may also augment the effectiveness of therapy for those with established illness.

KW - astaxanthin

KW - cAMP

KW - cGMP

KW - ginkgolide

KW - ibuprofen

KW - inflammation

UR - http://www.scopus.com/inward/record.url?scp=78650631519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650631519&partnerID=8YFLogxK

U2 - 10.1002/ptr.3160

DO - 10.1002/ptr.3160

M3 - Article

VL - 25

SP - 128

EP - 136

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 1

ER -