Sulphate metabolism of selenate-resistant Schizosaccharomyces pombe mutants

Luca Bánszky, Tibor Simonics, A. Maráz

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Selenate-resistant mutants were obtained from several strains of Schizosaccharomyces pombe. The obtained mutants all belonged to the same genetic complementation group. They were low in sulphate uptake activity and in ATP sulphurylase activity. They grew on medium containing sulphite, thiosulphate, cysteine or glutathione but not methionine as the sole source of sulphur. From these results, the mutants were concluded to carry mutations in the ATP sulphurylase gene. Inability of the mutants to utilize methionine as a sulphur source is rationalized by the absence of the reverse transsulphurylation pathway in this organism; wild type strains must utilize methionine as a sulphur source after it is degraded to give rise to sulphate.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalJournal of General and Applied Microbiology
Volume49
Issue number5
DOIs
Publication statusPublished - 2003

Fingerprint

Selenic Acid
Schizosaccharomyces
Sulfur
Methionine
Sulfates
Adenosine Triphosphate
Thiosulfates
Sulfites
Glutathione
Cysteine
Mutation
Genes

Keywords

  • ATP sulphurylase
  • Methionine degradation
  • Schizosaccharomyces pombe
  • Selenate resistance
  • Sulphate utilization

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Sulphate metabolism of selenate-resistant Schizosaccharomyces pombe mutants. / Bánszky, Luca; Simonics, Tibor; Maráz, A.

In: Journal of General and Applied Microbiology, Vol. 49, No. 5, 2003, p. 271-278.

Research output: Contribution to journalArticle

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