Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits

M. Kovács, G. Tornyos, Zs Matics, L. Kametler, V. Rajli, Zs Bodnár, M. Kulcsár, G. Huszenicza, Zs Keresztes, S. Cseh

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Abstract

Pannon White (n = 12) male rabbits (weight: 4050 to 4500 g, age: 9 months) received 2 ml of a suspension containing purified T-2 toxin by gavage for 3 days. The daily toxin intake was 4 mg/animal (0.78 to 0.99 mg/kg body weight (BW)). Control animals (n = 12) received toxin-free suspension for 3 days. Since a feed-refusal effect was observed on the second day after T-2 administration, a group of bucks (n = 10) were kept as controls (no toxin treatment) but on a restricted feeding schedule, that is, the same amount of feed was provided to them as was consumed by the exposed animals. On day 51 of the experiment (i.e. 48 days after the 3-day toxin treatment), semen was collected, and pH, concentration, motility and morphology of the spermatozoa, as well as concentration of citric acid, zinc and fructose in the seminal plasma, were measured. After gonadotropin-releasing hormone (GnRH) analogue treatment, the testosterone level was examined. One day of T-2 toxin treatment dramatically decreased voluntary feed intake (by 27% compared to control, P <0.05) and remained lower (P <0.05) during the first 2 weeks after the withdrawal of the toxin. BW of the contaminated rabbits decreased by 88% on days 17 and 29 compared to controls (P <0.05). No effect of toxin treatment was detected on pH and quantity of the semen or concentration of spermatozoa. The ratio of spermatozoa showing progressive forward motility decreased from 65% to 53% in the semen samples of toxin-treated animals compared to controls (P > 0.05). The ratio of spermatozoa with abnormal morphology increased (P <0.05) in the ejaculates collected from the toxin-treated animals. T-2 toxin applied in high doses decreased the concentration of citric acid in seminal plasma (P <0.05). No effect of T-2 toxin on the concentrations of the other seminal plasma parameters (fructose and zinc) was observed. T-2 toxin decreased the basic testosterone level by 45% compared to control (P <0.01) and resulted in lower (P <0.05) GnRH-induced testosterone concentration. Feed restriction, that is, less nutrient intake, resulted in more morphologically abnormal spermatozoa in the semen, but it did not cause significant loss in BW, motility of the spermatozoa, composition of the seminal plasma or testosterone concentration - its effect needs further examination.

Original languageEnglish
Pages (from-to)1563-1569
Number of pages7
JournalAnimal
Volume5
Issue number10
DOIs
Publication statusPublished - Oct 2011

Fingerprint

T-2 toxin
seminal plasma
testosterone
poisoning
toxins
rabbits
spermatozoa
restricted feeding
gonadotropin-releasing hormone
citric acid
fructose
semen
animals
zinc
synthetic hormones
voluntary intake
body weight
bucks
sperm concentration
sperm motility

Keywords

  • male
  • rabbit
  • spermatogenesis
  • T-2
  • testosterone production

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits. / Kovács, M.; Tornyos, G.; Matics, Zs; Kametler, L.; Rajli, V.; Bodnár, Zs; Kulcsár, M.; Huszenicza, G.; Keresztes, Zs; Cseh, S.

In: Animal, Vol. 5, No. 10, 10.2011, p. 1563-1569.

Research output: Contribution to journalArticle

Kovács, M. ; Tornyos, G. ; Matics, Zs ; Kametler, L. ; Rajli, V. ; Bodnár, Zs ; Kulcsár, M. ; Huszenicza, G. ; Keresztes, Zs ; Cseh, S. / Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits. In: Animal. 2011 ; Vol. 5, No. 10. pp. 1563-1569.
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abstract = "Pannon White (n = 12) male rabbits (weight: 4050 to 4500 g, age: 9 months) received 2 ml of a suspension containing purified T-2 toxin by gavage for 3 days. The daily toxin intake was 4 mg/animal (0.78 to 0.99 mg/kg body weight (BW)). Control animals (n = 12) received toxin-free suspension for 3 days. Since a feed-refusal effect was observed on the second day after T-2 administration, a group of bucks (n = 10) were kept as controls (no toxin treatment) but on a restricted feeding schedule, that is, the same amount of feed was provided to them as was consumed by the exposed animals. On day 51 of the experiment (i.e. 48 days after the 3-day toxin treatment), semen was collected, and pH, concentration, motility and morphology of the spermatozoa, as well as concentration of citric acid, zinc and fructose in the seminal plasma, were measured. After gonadotropin-releasing hormone (GnRH) analogue treatment, the testosterone level was examined. One day of T-2 toxin treatment dramatically decreased voluntary feed intake (by 27{\%} compared to control, P <0.05) and remained lower (P <0.05) during the first 2 weeks after the withdrawal of the toxin. BW of the contaminated rabbits decreased by 88{\%} on days 17 and 29 compared to controls (P <0.05). No effect of toxin treatment was detected on pH and quantity of the semen or concentration of spermatozoa. The ratio of spermatozoa showing progressive forward motility decreased from 65{\%} to 53{\%} in the semen samples of toxin-treated animals compared to controls (P > 0.05). The ratio of spermatozoa with abnormal morphology increased (P <0.05) in the ejaculates collected from the toxin-treated animals. T-2 toxin applied in high doses decreased the concentration of citric acid in seminal plasma (P <0.05). No effect of T-2 toxin on the concentrations of the other seminal plasma parameters (fructose and zinc) was observed. T-2 toxin decreased the basic testosterone level by 45{\%} compared to control (P <0.01) and resulted in lower (P <0.05) GnRH-induced testosterone concentration. Feed restriction, that is, less nutrient intake, resulted in more morphologically abnormal spermatozoa in the semen, but it did not cause significant loss in BW, motility of the spermatozoa, composition of the seminal plasma or testosterone concentration - its effect needs further examination.",
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AU - Kovács, M.

AU - Tornyos, G.

AU - Matics, Zs

AU - Kametler, L.

AU - Rajli, V.

AU - Bodnár, Zs

AU - Kulcsár, M.

AU - Huszenicza, G.

AU - Keresztes, Zs

AU - Cseh, S.

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N2 - Pannon White (n = 12) male rabbits (weight: 4050 to 4500 g, age: 9 months) received 2 ml of a suspension containing purified T-2 toxin by gavage for 3 days. The daily toxin intake was 4 mg/animal (0.78 to 0.99 mg/kg body weight (BW)). Control animals (n = 12) received toxin-free suspension for 3 days. Since a feed-refusal effect was observed on the second day after T-2 administration, a group of bucks (n = 10) were kept as controls (no toxin treatment) but on a restricted feeding schedule, that is, the same amount of feed was provided to them as was consumed by the exposed animals. On day 51 of the experiment (i.e. 48 days after the 3-day toxin treatment), semen was collected, and pH, concentration, motility and morphology of the spermatozoa, as well as concentration of citric acid, zinc and fructose in the seminal plasma, were measured. After gonadotropin-releasing hormone (GnRH) analogue treatment, the testosterone level was examined. One day of T-2 toxin treatment dramatically decreased voluntary feed intake (by 27% compared to control, P <0.05) and remained lower (P <0.05) during the first 2 weeks after the withdrawal of the toxin. BW of the contaminated rabbits decreased by 88% on days 17 and 29 compared to controls (P <0.05). No effect of toxin treatment was detected on pH and quantity of the semen or concentration of spermatozoa. The ratio of spermatozoa showing progressive forward motility decreased from 65% to 53% in the semen samples of toxin-treated animals compared to controls (P > 0.05). The ratio of spermatozoa with abnormal morphology increased (P <0.05) in the ejaculates collected from the toxin-treated animals. T-2 toxin applied in high doses decreased the concentration of citric acid in seminal plasma (P <0.05). No effect of T-2 toxin on the concentrations of the other seminal plasma parameters (fructose and zinc) was observed. T-2 toxin decreased the basic testosterone level by 45% compared to control (P <0.01) and resulted in lower (P <0.05) GnRH-induced testosterone concentration. Feed restriction, that is, less nutrient intake, resulted in more morphologically abnormal spermatozoa in the semen, but it did not cause significant loss in BW, motility of the spermatozoa, composition of the seminal plasma or testosterone concentration - its effect needs further examination.

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